Improved characterization of bispecific antibody chain mispairing by FabDELLO subunit digestion and LC-MS resolution of Fab variants.
Middle-level Analysis
Middle-level analysis involves analyzing defined protein subunits rather than intact proteins or small peptides. This approach provides a balance between simplicity and level of detail, enabling characterization of modifications and structural features. Our tools support controlled digestion and streamlined workflows for consistent middle-level analysis.
Applications
Fast and reliable middle-level analysis of antibody oxidation using FabRICATOR and GlycINATOR, supporting robust QC of critical quality attributes.
Above hinge digestion at a single lysine residue, producing defined fragments for robust LC-MS analysis of LALA-engineered IgG1 antibodies.
GlySERIAS Immobilized delivers a more complete digestion of linkers for precise characterization of fusion protein quality attributes.
Site-specific IgM digestion under physiological conditions, producing homogeneous fragments for precise middle-level LC-MS characterization.
Site-specific digestion of human IgA2m1, generating homogeneous Fab and Fc fragments for high-resolution middle-level LC-MS characterization.
Site-specific digestion of human IgA1, generating homogeneous Fab and Fc fragments for high-resolution middle-level LC-MS characterization.
Robust and reproducible digestion of dulaglutide linkers, separating GLP-1 peptides from the Fc for domain-specific LC-MS analysis of protein modifications
Efficient digestion of multi-domain BiTE molecules, separating the domains for high-quality LC-MS analysis and precise localization of protein modifications
Efficient digestion of hinge-mutated antibodies, generating fragments for comprehensive subunit LC-MS analysis of engineered IgG formats
Resources
How FabRICATOR® works
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FabRICATOR®
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SmartEnzymes™ for Digestion of Hinge-mutated Antibodies
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FabDELLO® for the Analysis of Hinge Mutated and Bispecific Antibodies
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SmartEnzymes™ Brochure
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