Applications

Here you find information about how you can use Genovis' products in your applications.

The SmartEnzyme Reference Tool organizes all papers published using our products. With this tool, you can filter by quality attribute, enzyme used, type of antibody molecule and technology.

Instructions for use

Product Instructions

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References

References

All references on Genovis' products

Smart Enzyme Reference Tool

SmartEnzyme Reference Tool

Find the right reference for the antibody attribute you are studying

Antibody Characterization

The Genovis enzymes have gained widespread use within the development and production of antibody-based therapeutics. Due to the high specificity and robustness of the enzymes, they are becoming an important tool for the ever-increasing demand of analytical characterization of large and complex biomolecules.

Analysis of O-glycans

Scientists at Genovis have discovered new O-glycan-specific enzymatic tools including: OpeRATOR™ - an O-glycan-specific endoprotease, OglyZOR™ - an O-glycosidase and SialEXO™ - a sialidase mix for removal of sialic acids. The enzymes allow detailed analysis of O-glycans.

Antibody Conjugation

The novel and unique conjugation technology GlyCLICK™ offers opportunities for site-specific conjugation of antibodies, using a two step enzymatic process and click-chemistry. GlyCLICK applications include e.g. ADC development, fluorescent imaging and in vivo imaging. GlyCLICK is a modular technology applicable to IgG from several species and subclasses and offers a robust and quantitative conjugation of 2 labels per antibody.

Generation of Antibody Fragments

Use FabRICATOR® to rapidly generate F(ab')2 and Fc/2 fragments from several species and subclasses of IgG, including human IgG1-4. Intact Fabs are easily generated from human IgG1 using the IgG-specific FabALACTICA™ enzyme or GingisKHAN®.

Deglycosylation of IgG

IgGZERO® (EndoS) and GlycINATOR® (EndoS2) rapidly hydrolyze N-glycans on the Fc domain of IgG. GlycINATOR removes all Fc glycoforms, including high-mannose and hybrid-type glycans. IgGZERO removes complex Fc glycoforms, but has limited activity on high-mannose and hybrid-type glycans.

IgG Protease Digestion Chart

With the IgG protease digestion chart, you see the digestion sites of the Genovis' enzymes and what species and subclass of antibody the enzymes digest.

Species & Subclasses FabRICATOR®
Native IgG
FabULOUS®
Digestion under reduced condition.
* Primary digestion site.
GingisKHAN®
Native IgG
Human IgG1 ..CPAPELLG / GPSVF.. ..KTHT / CPPCPAPEL..* ..KSCDK / THTCPPCP..
Human IgG2 ..CPAPPVA / GPSVF.. Yes (Site N.D.) X
Human IgG3 ..CPAPELLG / GPSVF.. Yes (Site N.D.) Multiple sites in Fc
Human IgG4 ..CPAPEFLG / GPSVF.. Yes, with longer incubation time X
Mouse IgG1 X ..KPCIC / TVPEVS.. X
Mouse IgG2a ..CPAPNLLG / GPSVF.. Yes (Site N.D.) X
Mouse IgG2b X Yes (Site N.D.) X
Mouse IgG3 ..CPPGNILG / GPSVF.. Yes (Site N.D.) X
Rat IgG2b ..CPVPELLG / GPSVF.. Yes (Several sites) N.D.
Rhesus Monkey ..CPAPELLG / GPSVF.. N.D. N.D.
Rabbit ..CPPPELLG / GPSVF.. ..KPT / CPPPE..* N.D.

X = no digestion confirmed
N.D. = Not determined

Latest References