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GingisREX™ – Arginine-specific Protein Digestion
GingisREX (RgpB) is an arginine-specific protease that digests proteins C-terminally of arginine residues, including Arg-Pro linkages that are difficult to digest with other enzymes.
SmartEnzymes™
GingisREX can be used in peptide mapping, de novo peptide sequencing and analysis of post-translational modifications.
Why GingisREX™?
- Specific digestion C-terminally of arginine residues
- Reliable digestion at the otherwise difficult arginine-proline sequences
- Generates longer peptides – improved sequence coverage
Digests any peptide or protein containing arginine, including Arg-Pro motifs
60 min reaction
Active in 6 M urea and 0.1% SDS
Digests C-terminally of arginine residues
Product Formats
About GingisREX™
GingisREX is a cysteine protease that specifically digests peptide bonds C-terminally to arginine residues, including arginine linked to proline. Cysteine is required for enzymatic activity.
Longer peptides are generated, as compared to trypsin digestion, which can be resolved and identified by high-resolution MS. This offers more options in sample preparation for bottom-up approaches, to achieve alternative digestion profiles and increased sequence coverage. GingisREX does not have activity at lysines, as commonly observed using Arg-C. The enzyme is active at a broad pH range of 5.0 – 9.0, it requires cysteine and is inhibited by guanidine hydrochloride.
The enzyme is purified from Porphyromonas gingivalis.
Applications
Arginine-specific peptide mapping with improved perfomance over Arg-C, generates larger peptides complementary to trypsin.
Performance Activity
Highly specific arginine-specific protease with limited off-target digestion, enabling clean peptide maps and high-confidence LC-MS data analysis.
Citations
Resources
GingisREX™ for Characterization of Lysine Conjugated ADCs
Download Scientific Poster
GlyCLICK® for Generation and Analysis of ADCs
Download Scientific Poster
GingisREX™ for Specific Digestion at Arginine Residues
Download Scientific Poster
FAQ and Support
Can guanidinium hydrochloride be used as a denaturing agent instead of urea?
No, GingisREX is inhibited by GdHCl even in low concentrations. This chaotrope should be avoided.
Is cysteine really needed for activation of the cysteine protease if TCEP or DTT is added in the reaction?
Yes, cysteine is required for both activity and specificity of the GingisREX enzyme.
Is the reaction ready in only 60 minutes?
The digestion time is affected by many factors like the enzyme to sample ratio, pH of the digestion reaction, and the sample characteristics. The reaction time needs to be optimized for each specific case.
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