FucosEXO®
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FucosEXO is a mix of α-Fucosidases for efficient hydrolysis of α1-2, α1-3 and α1-4-linked fucose residues on native N- and O-glycosylated proteins or free oligosaccharides.

SmartEnzymes™
FucosEXO is a valuable tool for glycan structure analysis on N- and O-glycoproteins and in exoglycosidase arrays on oligosaccharides.
Fucose on N- and O-glycosylated proteins
1-2 h reaction
No need for co-factors
Hydrolyzes ɑ1-2,3,4-linked fucose

Immobilized enzyme for defucosylation of glycoproteins in spin columns
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FucosEXO is a mix of α-Fucosidases for efficient hydrolysis of α1-2, α1-3 and α1-4-linked fucose on N- and O-glycosylated proteins or free oligosaccharides.
The enzymes in FucosEXO are expressed in E. coli with a His-tag, and have molecular weights of 87 kDa and 64 kDa. FucosEXO hydrolyzes fucose on glycoproteins under native conditions and displays high activity in a pH range from 6 to 8, without the need for co-factors or additives.
Rapid and complete defucosylation of O-glycans on TNFR proteins, outperforming alternative fucosidases across glycoengineered substrates.
Selective removal of α1-2/3/4 fucose for more comprehensive N- and O-glycan characterization.
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