TrypsINATOR™
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TrypsINATOR™ – Arginine- and Lysine-specific Protein Digestion
TrypsINATOR (Trypsin) is a serine protease that digests proteins C-terminally of arginine and lysine residues.
SmartEnzymes™
TrypsINATOR is recombinantly produced to high purity and is ideally suited for mass spectrometry-based proteomics applications. Additionally, the enzyme supports peptide mapping, de novo sequencing, and characterization of post-translational modifications.
TrypsINATOR is available as a single enzyme (TrypsINATOR Lyophilized) or pre-mixed in combination with LysCERATOR (TrypsINATOR LysCERATOR Mix Lyophilized).
Why TrypsINATOR™?
- Superior digestion efficiency with minimal missed cleavages
- Exceptional enzyme stability with minimal autoproteolysis
- Consistent digestion performance even during extended incubations
Digests peptides or proteins containing arginine and/or lysine residues
2 - 18 h reaction
Compatible with standard buffers including Tris-HCl, ammonium bicarbonate, HEPES, PBS and TEAB
Digests C-terminally of arginine and lysine residues
Product Formats
TrypsINATOR™ Lyophilized
Lyophilized enzyme for arginine- and lysine-specific protein digestion
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TrypsINATOR™ LysCERATOR Mix Lyophilized
Lyophilized enzyme mix for arginine- and lysine-specific protein digestion
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About TrypsINATOR™
TrypsINATOR (Trypsin) is a recombinantly produced serine protease that specifically digests peptide bonds C-terminally to arginine and lysine residues.
TrypsINATOR is highly efficient and facilitates consistent and specific cleavage while minimizing missed cleavages. TrypsINATOR is reductively methylated at lysine residues to minimize autoproteolysis, increase stability and ensure consistent performance, even during extended incubations. By combining TrypsINATOR with LysCERATOR, the TrypsINATOR LysCERATOR Mix offers enhanced digestion efficiency at lysine residues to further reduce missed cleavages, while offering improved digestion at challenging lysine-proline (K-P) sites. TrypsINATOR and TrypsINATOR LysCERATOR Mix are compatible with standard digestion buffers, including Tris-HCl, ammonium bicarbonate, HEPES, PBS and TEAB. TrypsINATOR is optimally active in a pH range of 7.0 – 9.0, and TrypsINATOR LysCERATOR Mix has an optimal pH range of 6.0 – 10.0.
Applications
Highly specific lysine and arginine digestion with more unique peptides and fewer missed cleavages for confident protein identification in complex proteomics workflows.
Performance Activity
Robust digestion performance with limited autoproteolysis, enabling cleaner LC–MS data and more confident peptide identification.
Improved digestion efficiency at challenging K–P sites compared to TrypsINATOR alone, resulting in more complete digestion with fewer missed cleavages.
Efficient digestion with minimal missed cleavages and high sequence coverage resulting in efficient peptide mapping and confident protein characterization.
FAQ and Support
Are TrypsINATOR and TrypsINATOR LysCERATOR Mix active at lower pH?
Yes, both TrypsINATOR and TrypsINATOR LysCERATOR Mix are active down to pH 5, however, with a lower digestion efficiency.
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