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OglyZOR™ – Hydrolysis of Core 1 O-glycans
OglyZOR is an endoglycosidase (O-Glycosidase) that specifically hydrolyzes core 1 O-glycans on native glycoproteins.
SmartEnzymes™
OglyZOR is used for removal of O-glycans for glycan analysis, confirmation of O-glycan presence and reduction of sample heterogeneity.
Why OglyZOR™?
- Highly efficient O-glycosidase
- Reduces sample heterogeneity to unmask potentially critical protein modifications
- Complete and robust removal of O-glycan core 1 disaccharides
O-glycans on glycoproteins
2-4h reaction
Requires removal of terminal sialic acids using SialEXO® (included)
Hydrolyzes core 1 type O-glycans
Product Formats
OglyZOR™ Lyophilized
Lyophilized enzyme for hydrolysis of core 1 O-glycans on glycoproteins
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About OglyZOR™
OglyZOR is an endoglycosidase (endo-α-N-Acetylgalactosaminidase) that catalyzes the hydrolysis of core 1, and to a limited extent core 3, O-glycans from native glycoproteins.
Removal of sialic acids is required for OglyZOR enzyme activity, and therefore, SialEXO Lyophilized composed of a mix of sialidases for removal of α2-3, α2-6 and α2-8-linked sialic acids, is supplied together with OglyZOR. Optimal activity is obtained at pH 6.5 to 7.5 and at 37°C.
OglyZOR is derived from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in E. coli. The SialEXO enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.
Performance Activity
Hydrolyzes core 1 O-glycans from native glycoproteins, supporting LC-MS workflows for accurate PTM mapping and protein characterization.
Efficient removal of core 1 O-glycans from complex glycoproteins to reduce heterogeneity and improve mass spectrometry characterization.
Citations
FAQ and Support
Can OglyZOR be used to deglycosylate peptides generated with OpeRATOR?
Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.
Will OglyZOR leave the N-glycans intact?
OglyZOR only works on desialylated O-glycans therefore is a sialidase (SialEXO) included with the product. SialEXO is not O-glycan specific and will therefore desialylate any N-glycans that are present.
Will OglyZOR work on extended core 1 structures or single HexNAc?
No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).
Can I use OglyZOR and PNGaseF in the same reaction?
Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions O/N at pH ~7.0. If denaturing reaction conditions are required for PNGaseF, the reaction with OglyZOR and SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
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