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GalNAcEXO® – Hydrolysis of α-linked GalNAcs
GalNAcEXO is an α-N-Acetylgalactosaminidase for efficient hydrolysis of terminal GalNAc residues on glycoproteins.
SmartEnzymes™
GalNAcs linked to serine or threonine, referred to as Tn antigen, are quickly and efficiently hydrolyzed by GalNAcEXO, and the enzyme also displays activity on α1-3-linked terminal GalNAcs.
The enzyme is a valuable tool that reduces sample heterogeneity for the analysis of complex O-glycoproteins that carry α-linked GalNAc residues as immature truncated core 1.
Why GalNAcEXO®?
- Highly efficient α-N-Acetylgalactosaminidase – fast and complete removal of Tn-antigen and α1-3-linked GalNAc
- Enables complete glycan removal for improved protein characterization
- Available immobilized in ready-to-use spin columns
α-linked GalNAc residues on O-glycoproteins
4 h reaction
No need for co-factors
Tn antigen and α1-3-linked terminal GalNAc
Product Formats
GalNAcEXO® Lyophilized
Lyophilized enzyme for hydrolysis of GalNAc residues on glycoproteins
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GalNAcEXO® Immobilized
Immobilized enzyme for hydrolysis of GalNAc residues on glycoproteins in spin columns
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About GalNAcEXO®
GalNAcEXO is an exo-α-N-Acetylgalactosaminidase for efficient hydrolysis of α-N-Acetylgalactosamine (GalNAc) linked to serine or threonine residues in glycoproteins (Tn antigen). The enzyme also displays activity on α1-3-linked terminal GalNAc.
GalNAcEXO hydrolyzes GalNAc on glycoproteins under native conditions and is highly active in the pH range 6.0 to 7.6. No co-factors or special buffers are required. The enzyme in GalNAcEXO is derived from Akkermansia muciniphila, expressed in E. coli with a His-tag, and has a molecular weight of 52 kDa.
Applications
Confirmation and quantification of Tn antigens on O-glycosylated biopharmaceuticals while simplifying intact-mass analysis and revealing protein variants.
O-glycan site occupancy profiling and complete deglycosylation of etanercept for in-depth characterization of complex O-glycoproteins.
Citations
Resources
SmartEnzymes™ for the Glycosylation Charcterization of Glycoproteins
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FAQ and Support
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