Description
GalNAcEXO is an exo-α-N-Acetylgalactosaminidase for efficient hydrolysis of α-N-acetylgalactosamine (GalNAc) linked to serine or threonine residues on glycoproteins (Tn antigen). The enzyme also displays activity on α1-3 linked terminal GalNAcs. GalNAcEXO hydrolyzes GalNAc on glycoproteins under native conditions and is highly active in the pH range 6.0 to 7.6. No cofactors or special buffers are required. The enzyme in GalNAcEXO is derived from Akkermansia muciniphila, expressed in E. coli with a His-tag, and has a molecular weight of 52 kDa.