FabDELLO® – Above Hinge Digestion of Human IgG1

FabDELLO is a protease that digests human IgG1 at a single site above the hinge, yielding intact Fab and Fc fragments within two hours, with no need for reducing conditions.

SmartEnzymes™

FabDELLO is active on antibodies with mutated hinge regions, such as the LALA mutation, and enables middle-level LC-MS for characterization of critical quality attributes on antibodies with mutated hinges.

Why FabDELLO®?

  • Robust digestion of hinge-mutated antibodies, including LALA-mutated antibodies
  • Fast – generates intact Fab and Fc fragments from human IgG1 within two hours
  • Middle-level characterization of human IgG1

  • Target

    Human IgG1, including antibodies with mutated hinge regions

  • Time

    2 hour reaction

  • Box

    Requires presence of calcium ions. 200 mM CaCl2 is included

  • Digestion Site

    …KSCDK / THTCPPCP… (above the hinge)

Product Formats

About FabDELLO®

FabDELLO digests human IgG1 at a single site above the hinge (…KSCDK / THTCPPCP…), generating intact Fab and Fc fragments.

FabDELLO is a lysine-specific protease. The single digestion site is a result of the three-dimensional structure of human IgG1 making this lysine exposed for the enzyme. Additional digestion sites at exposed lysines on the Fc may appear if the N-glycans are removed. FabDELLO is active under native conditions and requires the presence of calcium ions. Optimal activity is obtained at 37°C and pH 7-8.5.

FabDELLO is cloned from Bdellovibrio bacteriovorus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 32 kDa.

Applications

Performance Activity

Citations

Resources

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