Lyophilized FabDELLO for digestion of human IgG1. FabDELLO digests human IgG1 at a single amino acid site above the hinge region, generating intact Fab and Fc fragments. FabDELLO is active under native conditions and requires the presence of calcium ions (included).
| Application | Antibody Characterization, Antibody Fragmentation, Mutated IgG |
|---|---|
| Product Group | |
| Source/Origin | Cloned from B. Bacteriovorus, Produced in E. coli (His-tagged), 32 kDa |
| Product Composition | |
| Digestion Site | |
| Specificity | |
| Substrates | |
| Reaction Conditions | 2 hour reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
| Buffer Compatibility | Optimal activity is achieved in TBS buffer pH 7-8.5, Buffers containing phosphate should be avoided since the phosphate ions will form the insoluble calcium phosphate salt together with the calcium ions required for enzymatic activity |
| Additives/Co-factors | Requires the presence of calcium ions, 1 vial 20× CaCl2 solution (200mM) is included |
| Unit Definition |
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