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IgMBRAZOR™ – Digestion of IgM
IgMBRAZOR is a unique IgM-specific protease that digests human IgM at one specific site below the CH2 domain in the heavy chain, generating homogeneous F(ab’)2 and Fc fragments.
SmartEnzymes™
IgMBRAZOR enables middle-level analysis of the complex and high molecular weight human IgM, which facilitates IgM characterization during, for example, the development of vaccines and diagnostics.
Why IgMBRAZOR™?
- Unique – enables middle-level characterization and quality control of IgM
- Fast – generates homogeneous F(ab’)2 and Fc fragments within 30 minutes
- Highly specific – one digestion site in the heavy chain of human IgM
Human IgM
30 min reaction
No need for reducing agents or co-factors
VPDQDT / AIRVFA (in the heavy chain)
Product Formats
About IgMBRAZOR™
IgMBRAZOR is a unique IgM-specific enzyme that digests human IgM at one specific site below the CH2 domain in the heavy chain, generating F(ab’)2 and Fc fragments.
The digestion is complete within 30 minutes, and due to the specificity of the enzyme, there is no risk of overdigestion if the incubation time is prolonged. The enzyme is active at pH 5.5 to 9.0 and does not require reducing conditions or co-factors for activity. The optimal activity is at 37°C, but the digestion can also be performed at room temperature using an increased incubation time.
IgMBRAZOR is expressed in E. coli. The enzyme contains a His-tag and has a molecular weight of 38 kDa.
Applications
Site-specific IgM digestion under physiological conditions, producing homogeneous fragments for precise middle-level LC-MS characterization.
Isotype-specific digestion of IgM and IgG in serum, allowing simultaneous analysis without enzymatic cross-interference.
Performance Activity
IgM-specific digestion with no off-target cleavage of IgG or IgA, enabling selective generation of Fab’ and Fc fragments.
Citations
FAQ and Support
Does IgMBRAZOR digest only human IgM?
IgM from some species of monkey may also be digested by IgMBRAZOR.
Which buffers are compatible with IgMBRAZOR?
The enzyme is active at pH 5.5 to 9.0, tolerates up to 300 mM NaCl, and does not require reducing conditions or co-factors for activity. PBS and TBS buffers are both compatible. Optimization may be required if buffers other than the recommended are used.
Can IgMBRAZOR and PNGase F be used in the same reaction?
Yes, IgMBRAZOR and PNGase F can be used in combination if the reaction is performed under non-denaturing conditions. However, for complete deglycosylation of IgM using PNGase F denaturing conditions may be required and it is then recommended to perform the IgMBRAZOR digestion prior the PNGase F deglycosylation.
Can IgMBRAZOR and FabRICATOR be used in the same reaction?
Yes, IgMBRAZOR and FabRICATOR can be used in combination.
Is it possible to use IgMBRAZOR with very low concentrations of IgM?
It is possible to use IgMBRAZOR with low concentrations of IgM. However, this will lead to a decreased reaction rate, so for IgM concentrations below 0.5 mg/mL it is recommended to increase the amount of added enzyme and/or increase the incubation time. There is no risk of overdigestion and the incubation time can be prolonged to overnight if necessary.
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