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SialEXO™ – Hydrolysis of Sialic Acids
The SialEXO products are sialidases for the removal and analysis of sialic acids. The enzymes are active on both N- and O-linked glycans present on native glycoproteins or released glycan structures.
SmartEnzymes™
SialEXO is used to pretreat O-glycosylated proteins prior to digestion with OpeRATOR or OglyZOR. Other applications include reduction of sample complexity, charge variant analyses and exoglycosidase arrays.
Why SialEXO™?
- Efficient desialylation of N-linked, O-linked and released glycans
- Fast removal of sialic acids for enhanced analysis of protein charge variants
- Available immobilized in ready-to-use spin columns
Sialic acids on N- and O-linked glycans
30 min - 2 h reaction
No need for co-factors
α2-3, α2-6 and α2-8-linked sialic acids
Product Formats
SialEXO™ Immobilized
Immobilized enzymes for desialylation of glycoproteins in spin columns
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About SialEXO™
The SialEXO products are sialidases for efficient desialylation of N- and O-glycosylated proteins.
SialEXO Lyophilized consists of two sialidases, each having unique activities and they work simultaneously. One enzyme has a broad activity for α2-3, α2-6 and α2-8-linked sialic acids, and the other one (SialEXO 2-3) quickly hydrolyzes sialic acids with α2-3-linkage. SialEXO Lyophilized removes all sialic acids on native proteins within 2 h.
SialEXO hydrolyzes glycoproteins under native conditions and displays activity in pH ranging from 6.5 to 9.
The enzymes are derived from Akkermansia muciniphila and expressed in E. coli. Both enzymes contain a His-tag and the molecular weights of the enzymes are 43 kDa and 66 kDa.
Applications
Simplified charge variant analysis by removing sialic acid-driven heterogeneity to reveal underlying protein profile.
Performance Activity
Complete desialylation of complex glycoproteins to simplify glycan profiles and enable more confident protein characterization.
Citations
Resources
SmartEnzymes™ for the Glycosylation Charcterization of Glycoproteins
Download Scientific Poster
SialEXO™ for Complete and Rapid Desialylation of Therapeutic Glycoproteins
Download Scientific Poster
SmartEnzymes™ for O-glycosylation Analysis using LC-MS
Download Scientific Poster
FAQ and Support
Can I use SialEXO in combination with OglyZOR or OpeRATOR?
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Can I use SialEXO for desialylation of both N- and O-glycans?
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
Does SialEXO contain a purification tag?
Yes, SialEXO contains a 6x His-tag.
Can I use SialEXO in combination with PNGaseF?
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
Does SialEXO require denaturation of the proteins for activity on glycoproteins?
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
Legal & Disclaimers
All rights reserved. Genovis products may be covered by one or more patents, trademarks and copyrights owned by Genovis AB or licensed from third parties. For more information about commercial rights, please contact the Genovis team at licensing@genovis.com.
Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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