SialEXO™
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SialEXO™ 2-3 Lyophilized
Lyophilized enzyme for hydrolysis of ɑ2-3-linked sialic acids
The SialEXO products are sialidases for the removal and analysis of sialic acids. The enzymes are active on both N- and O-linked glycans present on native glycoproteins or released glycan structures.
SialEXO 2-3 Lyophilized is available as a lyophilized powder in 500 unit vials for desialylation of 0.5 mg glycoprotein.
In contrast to SialEXO, which is a sialidase mix, SialEXO 2-3 is an α2-3 specific sialidase, allowing targeted analysis of α2-3 linked sialic acids only. Efficient α2-3 desialylation of O- and N-glycosylated proteins can be achieved within 1 h.
SialEXO 2-3 hydrolyzes glycoproteins under native conditions and displays activity in pH ranging from 7.0 to 9.0. The enzyme is derived from Akkermansia muciniphila and expressed in E. coli. The enzyme has a His-tag and the molecular weight is 66 kDa.
Why SialEXO™ 2-3 Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
SialEXO™ 2-3 Lyophilized 500 units
Lyophilized enzyme for hydrolysis of ɑ2-3-linked sialic acids from 0.5 mg glycoprotein
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit of SialEXO 2-3 Lyophilized hydrolyzes α2-3 linked sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 7.5 at 37 °C for 1 h.
Content and Storage
SialEXO 2-3 Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
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FAQ and Support
Can I use SialEXO in combination with PNGaseF?
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
Can I use SialEXO for desialylation of both N- and O-glycans?
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
Can I use SialEXO in combination with OglyZOR or OpeRATOR?
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Does SialEXO contain a purification tag?
Yes, SialEXO contains a 6x His-tag.
Does SialEXO require denaturation of the proteins for activity on glycoproteins?
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
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