Genovis Product Portfolio
Genovis provides a range of SmartEnzymes with specific enzymatic activities, a variety of research antibodies, a conjugation technology for site-specific antibody labeling and a platform method for specific IgG glycan remodeling.
The applications of SmartEnzymes include antibody digestion, antibody deglycosylation, antibody conjugation, N- and O-glycan profiling, affinity purification and proteomics. Additionally, we offer custom conjugation and antibody services, including fragmentation services and custom antibody development, production and purification.
FabRICATOR (IdeS)
Below hinge digestion of IgG.
FabRICATOR Z (IdeZ)
Below hinge digestion of mouse IgG.
FabULOUS (SpeB)
Above hinge digestion of IgG.
GlySERIAS
Hydrolysis of Flexible Linkers.
FabDELLO
Above hinge digestion of human IgG1.
FabALACTICA (IgdE)
Above hinge digestion of human IgG1.
GingisKHAN (Kgp)
Above hinge digestion of human IgG1.
Antibody Digestion
Our IgG proteases have specific enzymatic activities and digest IgG from several species and subclasses to generate homogenous subunits that facilitate analytical or preparative workflows for the characterization, development and production of biopharmaceuticals.
The middle-level analytical approach is used to e.g. identify and analyze critical quality attributes, including post-translational modifications such as glycosylation and oxidation. Moreover, the enzymes can be applied in quality control and stability testing of biopharmaceuticals.
For the preparation and purification of IgG fragments such as intact Fabs, F(ab’)2 and Fc, we provide easy-to-use kit solutions.
GlycINATOR (EndoS2)
Hydrolysis of all Fc-glycans.
IgGZERO (EndoS)
Hydrolysis of complex Fc-glycans.
Antibody Deglycosylation
The IgG-specific glycosidases trim IgG Fc-glycoforms on native antibodies, including high-mannose, hybrid, complex, and bisecting type glycans. The enzymes are used to reduce the complexity of antibody-based therapeutics and to study core afucosylation and N-glycan site occupancy.
GlyCLICK
Site-specific conjugation of native IgG.
TransGLYCIT
Transglycosylation of IgG.
Antibody Conjugation
The GlyCLICK technology is a platform for site-specific labeling at the Fc-glycan sites of native IgG, and it consists of a combination of enzymatic glycan remodeling and click chemistry. The technology is available in a range of kit formats to facilitate tailored conjugates using customized labels for bioassays, ADC development and in vitro or in vivo imaging. TransGLYCIT is a platform that enables efficient and site-specific human IgG glycan remodeling and conjugation. With TransGLYCIT, antibodies with defined and homogenous glycoforms or site-specific azide-activation are generated using fast and robust enzymatic workflows.
OmniGLYZOR
Hydrolysis of N- and mucin-type O-glycans.
GalactEXO
Hydrolysis of β1-3,4 linked galactose.
GalNAcEXO
Hydrolysis of α-linked GalNAcs.
PNGase F
Hydrolysis of N-glycans.
Glycan Profiling
The glycan profiling enzymes facilitate the structural analysis of a protein’s glycoform. We provide endo- and exoglycosidases that trim N- and O-glycans, and enzymatic tools for specific digestion of O-glycoproteins at the O-glycan sites. Together, the enzymes enable middle-down analytical approaches for O-glycosylated proteins and are applied in O-glycan profiling and site determination, in Tn antigen characterization and in reducing sample heterogeneity.
GlycOCATCH
Enrichment of O-glycopeptides.
Affinity Purification
The tool for affinity purification of O-glycosylated proteins and peptides is used to enrich proteins and peptides carrying O-glycans from samples, including complex samples such as human serum.
GingisREX (RgpB)
Arginine-specific protein digestion.
Proteomics
Proteases are widely used to generate peptides for analysis of proteins with mass spectrometry. Our arginine-specific protease has applications in peptide mapping, protein sequence analysis, protein fingerprinting and analysis of post-translational modifications.
Anti-FabRICATOR
Antibody (Anti-IdeS) detecting the FabRICATOR enzyme.
QED Antibodies
Mono- and polyclonal antibodies.
Research Antibodies
We provide a wide range of monoclonal and polyclonal antibodies with applications in cancer research, infectious disease diagnostics, therapeutic drug monitoring and cell biology. We also offer an antibody for the detection of the FabRICATOR (IdeS) enzyme in IHC, Western Blot and ELISA assays, as well as in preclinical gene therapy applications.
Custom Services
We offer custom fragmentation, conjugation and antibody services. We have expertise in generating, purifying and analyzing various fragments from different species and subclasses of IgG, and we also develop optimized protocols for digestion, purification and analysis. Our conjugation services include site-specific labeling of IgG with a label of your choice, and analysis to verify the degree of labeling (DOL).
The custom antibody services include monoclonal and polyclonal antibody development, in vitro monoclonal antibody production, anti-idiotype antibody development and antibody purification.
We also offer fast and accurate LC-MS profiling of human IgG using unique sample preparation based on SmartEnzymes workflows. Our SmartEnzymes enable middle-level LC-MS analysis of antibodies to characterize critical quality attributes such as the glycosylation profile and mass confirmation.