Genovis Product Portfolio

Genovis provides a range of SmartEnzymes with specific enzymatic activities, a variety of research antibodies and a conjugation technology for site-specific antibody labeling.
The applications of SmartEnzymes include antibody digestion, antibody deglycosylation, N- and O-glycan profiling, affinity purification and proteomics. Additionally, we offer custom conjugation and antibody services, including fragmentation services and custom antibody development, production and purification.

FabRICATOR (IdeS)

Below hinge digestion of IgG.

FabRICATOR Z (IdeZ)

Below hinge digestion of mouse IgG.

FabULOUS (SpeB)

Above hinge digestion of IgG.

FabALACTICA (IgdE)

Above hinge digestion of human IgG1.

GingisKHAN (Kgp)

Above hinge digestion of human IgG1.

Antibody Digestion

Our IgG proteases have specific enzymatic activities and digest IgG from several species and subclasses to generate homogenous subunits that facilitate analytical or preparative workflows for the characterization, development and production of biopharmaceuticals.
The middle-level analytical approach is used to e.g. identify and analyze critical quality attributes, including post-translational modifications such as glycosylation and oxidation. Moreover, the enzymes can be applied in quality control and stability testing of biopharmaceuticals.
For the preparation and purification of IgG fragments such as intact Fabs, F(ab’)2 and Fc, we provide easy-to-use kit solutions.   

GlycINATOR (EndoS2)

Hydrolysis of all Fc-glycans.

IgGZERO (EndoS)

Hydrolysis of complex Fc-glycans.

Antibody Deglycosylation

The IgG-specific glycosidases trim IgG Fc-glycoforms on native antibodies, including high-mannose, hybrid, complex, and bisecting type glycans. The enzymes are used to reduce the complexity of antibody-based therapeutics and to study core afucosylation and N-glycan site occupancy.

GlyCLICK

Site-specific conjugation of native IgG.

Antibody Conjugation

The GlyCLICK technology is a platform for site-specific labeling at the Fc-glycan sites of native IgG, and it consists of a combination of enzymatic glycan remodeling and click chemistry. The technology is available in a range of kit formats to facilitate tailored conjugates using customized labels for bioassays, ADC development and in vitro or in vivo imaging.

OpeRATOR

O-glycan specific protein digestion.

OglyZOR

Hydrolysis of core-1 O-glycans.

SialEXO

Hydrolysis of sialic acids.

FucosEXO

Hydrolysis of α1-2,3,4 linked fucose.

GalactEXO

Hydrolysis of β1-3,4 linked galactose.

GalNAcEXO

Hydrolysis of α-linked GalNAcs.

Glycan Profiling

The glycan profiling enzymes facilitate the structural analysis of a protein’s glycoform. We provide endo- and exoglycosidases that trim N- and O-glycans, and enzymatic tools for specific digestion of O-glycoproteins at the O-glycan sites. Together, the enzymes enable middle-down analytical approaches for O-glycosylated proteins and are applied in O-glycan profiling and site determination, in Tn antigen characterization and in reducing sample heterogeneity.

GlycOCATCH

Enrichment of O-glycopeptides.

Affinity Purification

The tool for affinity purification of O-glycosylated proteins and peptides is used to enrich proteins and peptides carrying O-glycans from samples, including complex samples such as human serum.

GingisREX (RgpB)

Arginine-specific protein digestion.

Proteomics

Proteases are widely used to generate peptides for analysis of proteins with mass spectrometry. Our arginine-specific protease has applications in peptide mapping, protein sequence analysis, protein fingerprinting and analysis of post-translational modifications.

Anti-FabRICATOR

Antibody (Anti-IdeS) detecting the FabRICATOR enzyme.

QED Antibodies

Mono- and polyclonal antibodies.

Research Antibodies

We provide a wide range of monoclonal and polyclonal antibodies with applications in cancer research, infectious disease diagnostics, therapeutic drug monitoring and cell biology. We also offer an antibody for the detection of the FabRICATOR (IdeS) enzyme in IHC, Western Blot and ELISA assays, as well as in preclinical gene therapy applications.

Custom Services

We offer custom fragmentation, conjugation and antibody services. We have expertise in generating, purifying and analyzing various fragments from different species and subclasses of IgG, and we also develop optimized protocols for digestion, purification and analysis. Our conjugation services include site-specific labeling of IgG with a label of your choice, and analysis to verify the degree of labeling (DOL).
The custom antibody services include monoclonal and polyclonal antibody development, in vitro monoclonal antibody production, anti-idiotype antibody development and antibody purification.

Order