Our SmartEnzymes™ Portfolio

Genovis SmartEnzymes portfolio consists of IgG Proteases, IgG Glycosidases, Enzymes for O-Glycans, Exoglycosidases and Conjugation.

Nature offers a vast source of enzymes, perfected through evolution to perform defined reactions. At Genovis, we believe that enzymes with unique properties can be used as biological tools to support the research and development of complex biopharmaceuticals to help bring safe and effective medicines to patients in need. Our task is to identify new enzymes and give them names. 

We call them SmartEnzymes.

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IgG Glycosidases

Conjugation

SialEXO 23

GalactEXO

GalNAcEXO

Proteases

IgG PROTEASES

Our IgG Proteases SmartEnzymes portfolio consists of FabRICATOR® (IdeS protease), FabRICATOR®Z (IdeZ), FabALACTICA® (IgdE)FabULOUS® (SpeB), GingisKHAN® (KGP).

The general protease GingisREX® (RgpB) is also included. The enzymes are applied in analytical workflows of biotherapeutic proteins, including antibody subunit analysis using liquid chromatography and mass spectrometry.

More information can be found on our Applications page or by downloading our Applications notes or Posters.

SmartEnzymes FabRICATOR (IdeS) info

Fabricator SmartEnzymes

  • Human IgG1-4, IgG of some classes from monkey, rat, rabbit and sheep
  • 30 min reaction
  • No need for reducing agents or co-factors
  • CPAPELLG / GPSVF (below the hinge)

SmartEnzymes GingisKHAN (KGP) info

Gingiskhan SmartEnzymes

  • Human IgG1
  • 60 min reaction
  • 2 mM cysteine (included)
  • KSCDK / THTCPPCP (above the hinge)

 

 SmartEnzymes FabRICATOR Z (IdeZ) info

FabricatorZ SmartEnzymes

  • Mouse IgG2a and IgG3, IgG from monkey, rabbit and sheep
  • 2 h reaction
  • No need for reducing agents or co-factors
  • CPAPNLLG / GPSVF (below the hinge)

SmartEnzymes FabULOUS (SpeB) info

Fabulous SmartEnzymes

  • Human IgG1-4, IgG from mouse, rat, goat, sheep and rabbit
  • 60 min reaction
  • Requires reducing conditions
  • Human IgG1: KTHT / CPPCPAP (above the hinge)

 SmartEnzymes Fabalactica info

fabalactica SmartEnzymes

  • Human IgG1
  • Overnight (O/N) reaction
  • No need for reducing agents or co-factors
  • KSCDKT / HTCPPCP (above the hinge)

Gingisrex SmartEnzymes

  • Digests any peptide or protein containing arginine. Specific for Arg-X motifs
  • 60 min reaction
  • Active in 6 M urea and 0.1% SDS
  • C-terminally of arginine residues

IgG GLYCOSIDASES & CONJUGATION

The Genovis product line of IgG Glycosidases and Conjugation consists of IgGZERO® (EndoS), GlycINATOR® (EndoS2) and GlyCLICK® (Conjugation).

More information can be found on our Applications page or by downloading our Applications notes or Posters.

SmartEnzymes glyclick poster

SmartEnzymes IgGZERO (EndoS) info

IgGZERO SmartEnzymes

  • Human IgG1-4, Fc-fusion proteins, IgG from mouse, rat, monkey, sheep, goat, cow and horse
  • 30 min reaction
  • Requires native IgG fold
  • Complex Fc glycoforms, limited activity on high-mannose and hybrid-type glycans

SmartEnzymes GlycINATOR (EndoS2) info

Glycinator SmartEnzymes

  • Human IgG1-4, Fc-fusion proteins, IgG from mouse, rabbit, rat, monkey, sheep, goat, cow and horse
  • 30 min reaction
  • Requires native IgG fold
  • All Fc glycoforms of IgG

SmartEnzymes GlyCLICK info

glyclick SmartEnzymes

  • Human IgG1-4, Fc-fusion proteins, IgG from mouse, rabbit, rat, monkey, sheep, goat, cow and horse
  • ~2-3 day protocol
  • Available labels: AlexaFluor®488, biotin and DFO. Azide activation kits are available for custom conjugation

SmartEnzymes for O-glycans & EXOglycosidases

The Genovis product line of enzymes for O-glycans consists of OpeRATOR® (O-glycan-specific protease), GlycOCATCH® (enrichment resin for O-glycosylated proteins and peptides), and OglyZOR® (O-glycosidase). The recently launched EXOglycosidases include GalactEXO (galactosidase), GalNAcEXO (exo-α-N-Acetylgalactosaminidase) and SialEXO® (sialidase).

More information can be found on our Applications page or by downloading our Applications notes or Posters.

operator application note

 SmartEnzymes Operator info

Operator SmartEnzymes

  • Digests native mucin-type O-glycosylated proteins
  • 2h to overnight (16-18h) reaction
  • Desialylation using SialEXO® (included) increases performance
  • N-terminally of O-glycosylated serine and threonine residues

 SmartEnzymes SialEXO info

Sialexo SmartEnzymes

  • Hydrolyzes sialic acids on N- and O-linked glycans
  • α2-3, α2-6 and α2-8-linked sialic acids
  • 30 min - 2 h reaction
  • Requires no co-factors

 SmartEnzymes Glycocatch info

Glycocatch SmartEnzymes

  • Binds mucin-type O-glycosylated proteins and peptides
  • 2h binding
  • Desialylation using SialEXO® (included) increases binding
  • Glycoproteins and peptides carrying mucin-type O-glycans

 SmartEnzymes GalactEXO info

Galactexo SmartEnzymes

  • Galactosidase hydrolyzing galactose on N- and O-glycoproteins or free oligosaccharides
  • β1-3 and β1-4 linked galactose residues
  • 2 h reaction
  • Requires no co-factors

 SmartEnzymes Oglyzor info

Olgyzor SmartEnzymes

  • Hydrolyzes O-glycans on glycoproteins
  • 2-4h reaction
  • Requires removal of sialic acids using SialEXO® (included)
  • Core 1 type O-glycan disaccharides

  • α-N-Acetylgalactosaminidase hydrolyzing GalNAcs on O-glycoproteins
  • Tn antigen (core GalNAc) and α1-3 terminal GalNAc
  • 4 h reaction
  • No co-factors required

Applications

Learn more about the applications of Genovis SmartEnzymes.

Link to store GlyCLICK

Online Store

Buy the Genovis Enzymes for Biopharmaceuticals online using a credit card

Resources

Download Genovis brochures and posters with the latest details on the SmartEnzymes

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Meet us online and tell us more about your work to find the perfect SmartEnzyme for your application!

Services

We offer services for your antibody fragmentation project.  In addition to our in-house produced SmartEnzymes product portfolio, we also use other classic enzymes in our service. Moreover, we have extensive experience purifying and analyzing the final fragments.

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