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- Human IgG1-4, IgG of some classes from monkey, rat, rabbit and sheep
- 30 min reaction
- No need for reducing agents or co-factors
- CPAPELLG / GPSVF (below the hinge)
- Human IgG1
- 60 min reaction
- 2 mM cysteine (included)
- KSCDK / THTCPPCP (above the hinge)
- Mouse IgG2a and IgG3, IgG from monkey, rabbit and sheep
- 2 h reaction
- No need for reducing agents or co-factors
- CPAPNLLG / GPSVF (below the hinge)
- Human IgG1-4, IgG from mouse, rat, goat, sheep and rabbit
- 60 min reaction
- Requires reducing conditions
- Human IgG1: KTHT / CPPCPAP (above the hinge)
- Human IgG1
- Overnight (O/N) reaction
- No need for reducing agents or co-factors
- KSCDKT / HTCPPCP (above the hinge)
- Digests any peptide or protein containing arginine. Specific for Arg-X motifs
- 60 min reaction
- Active in 6 M urea and 0.1% SDS
- C-terminally of arginine residues
- Human IgG1-4, Fc-fusion proteins, IgG from mouse, rat, monkey, sheep, goat, cow and horse
- 30 min reaction
- Requires native IgG fold
- Complex Fc glycoforms, limited activity on high-mannose and hybrid-type glycans
- Human IgG1-4, Fc-fusion proteins, IgG from mouse, rabbit, rat, monkey, sheep, goat, cow and horse
- 30 min reaction
- Requires native IgG fold
- All Fc glycoforms of IgG
- Human IgG1-4, Fc-fusion proteins, IgG from mouse, rabbit, rat, monkey, sheep, goat, cow and horse
- ~2-3 day protocol
- Available labels: AlexaFluor®488, biotin and DFO. Azide activation kits are available for custom conjugation
- Digests native mucin-type O-glycosylated proteins
- 2h to overnight (16-18h) reaction
- Desialylation using SialEXO® (included) increases performance
- N-terminally of O-glycosylated serine and threonine residues
- Hydrolyzes sialic acids on N- and O-linked glycans
- α2-3, α2-6 and α2-8-linked sialic acids
- 30 min - 2 h reaction
- Requires no co-factors
- Binds mucin-type O-glycosylated proteins and peptides
- 2h binding
- Desialylation using SialEXO® (included) increases binding
- Glycoproteins and peptides carrying mucin-type O-glycans
- Galactosidase hydrolyzing galactose on N- and O-glycoproteins or free oligosaccharides
- β1-3 and β1-4 linked galactose residues
- 2 h reaction
- Requires no co-factors
- Hydrolyzes O-glycans on glycoproteins
- 2-4h reaction
- Requires removal of sialic acids using SialEXO® (included)
- Core 1 type O-glycan disaccharides
- α-N-Acetylgalactosaminidase hydrolyzing GalNAcs on O-glycoproteins
- Tn antigen (core GalNAc) and α1-3 terminal GalNAc
- 4 h reaction
- No co-factors required
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