SialEXO™
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The SialEXO products are sialidases for the removal and analysis of sialic acids. The enzymes are active on both N- and O-linked glycans present on native glycoproteins or released glycan structures.
SialEXO Lyophilized is available as a lyophilized powder in 2000 unit vials for desialylation of 2 mg glycoprotein.
Why SialEXO™ Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
SialEXO™ Lyophilized 2000 units
Lyophilized enzyme mix for desialylation of 2 mg glycoprotein
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit of SialEXO Lyophilized hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.
Content and Storage
SialEXO Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
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SmartEnzymes™ for O-glycosylation Analysis using LC-MS
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Applications
Simplified charge variant analysis by removing sialic acid-driven heterogeneity to reveal underlying protein profile.
Performance Activity
Complete desialylation of complex glycoproteins to simplify glycan profiles and enable more confident protein characterization.
FAQ and Support
Does SialEXO contain a purification tag?
Yes, SialEXO contains a 6x His-tag.
Can I use SialEXO in combination with OglyZOR or OpeRATOR?
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Does SialEXO require denaturation of the proteins for activity on glycoproteins?
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
Can I use SialEXO in combination with PNGaseF?
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
Can I use SialEXO for desialylation of both N- and O-glycans?
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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