€506.00 – €1,782.00
Immobilized SialEXO rapidly desialylates glycoproteins without residual enzyme in the final preparation
Immobilized SialEXO contains spin columns with immobilized SialEXO on agarose beads. The immobilized enzyme acts on α2-3, α2-6 and α2-8 linked sialic acids for complete desialylation of N- and O-linked glycans on native (non-denatured) proteins and peptides within 30 min. Immobilized SialEXO is available in spin columns for desialylation of 0.5 mg of sialylated glycoproteins without any residual enzyme in the final preparation.
| Application | |
|---|---|
| Product Group | |
| Source/Origin | Cloned from A. muciniphila, Produced in E. coli (His-tagged), 43 kDa, 66 kDa |
| Product Composition | |
| Digestion Site | |
| Specificity | |
| Substrates | |
| Reaction Conditions | 30 minute reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
| Buffer Compatibility | Other buffers or pH (6.5 – 9.0) can be used but the reaction conditions need to be optimized. |
| Inhibitors | |
| Unit Definition | 0.1 ml settled resin desialylates ≥ 90% sialic acids of 0.5 mg glycoprotein (fetuin) |
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.
Yes, SialEXO contains a 6x His-tag.
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
