IgMBRAZOR™
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IgMBRAZOR is a unique IgM-specific protease that digests human IgM at one specific site below the CH2 domain in the heavy chain, generating homogeneous F(ab’)2 and Fc fragments.
IgMBRAZOR Lyophilized is available as a lyophilized powder in 1000 unit vials for digestion of 1 mg human IgM.
Why IgMBRAZOR™ Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
IgMBRAZOR™ Lyophilized 1000 units
Lyophilized enzyme for digestion of 1 mg IgM
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit IgMBRAZOR digests ≥90% of 1 µg human myeloma IgM when incubated in PBS (10 mM sodium phosphate, 140 mM NaCl, 2.7 mM KCl), pH 7.4 at room temperature for 20 minutes.
Content and Storage
IgMBRAZOR Lyophilized is reconstituted by the addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped cold, and should be stored at -20°C upon arrival.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
Site-specific IgM digestion under physiological conditions, producing homogeneous fragments for precise middle-level LC-MS characterization.
Isotype-specific digestion of IgM and IgG in serum, allowing simultaneous analysis without enzymatic cross-interference.
Performance Activity
IgM-specific digestion with no off-target cleavage of IgG or IgA, enabling selective generation of Fab’ and Fc fragments.
Citations
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Can IgMBRAZOR and FabRICATOR be used in the same reaction?
Yes, IgMBRAZOR and FabRICATOR can be used in combination.
Is it possible to use IgMBRAZOR with very low concentrations of IgM?
It is possible to use IgMBRAZOR with low concentrations of IgM. However, this will lead to a decreased reaction rate, so for IgM concentrations below 0.5 mg/mL it is recommended to increase the amount of added enzyme and/or increase the incubation time. There is no risk of overdigestion and the incubation time can be prolonged to overnight if necessary.
Does IgMBRAZOR digest only human IgM?
IgM from some species of monkey may also be digested by IgMBRAZOR.
Which buffers are compatible with IgMBRAZOR?
The enzyme is active at pH 5.5 to 9.0, tolerates up to 300 mM NaCl, and does not require reducing conditions or co-factors for activity. PBS and TBS buffers are both compatible. Optimization may be required if buffers other than the recommended are used.
Can IgMBRAZOR and PNGase F be used in the same reaction?
Yes, IgMBRAZOR and PNGase F can be used in combination if the reaction is performed under non-denaturing conditions. However, for complete deglycosylation of IgM using PNGase F denaturing conditions may be required and it is then recommended to perform the IgMBRAZOR digestion prior the PNGase F deglycosylation.
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