GalNAcEXO®
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GalNAcEXO® Immobilized
Immobilized enzyme for hydrolysis of GalNAc residues on glycoproteins in spin columns
GalNAcEXO is an α-N-Acetylgalactosaminidase for efficient hydrolysis of terminal GalNAc residues on glycoproteins.
The GalNAcEXO Immobilized spin columns contain GalNAcEXO enzyme covalently coupled to agarose beads, for hydrolysis of GalNAc residues on glycoproteins without contaminating the final preparation with enzyme.
The Microspin columns are available for hydrolysis of 5 or 10 x 0.5 mg glycoprotein.
Why GalNAcEXO® Immobilized?
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
GalNAcEXO® Immobilized Microspin 5 x 0.5 mg
Immobilized enzyme for hydrolysis of GalNAc residues on 5 x 0.5 mg glycoprotein in spin columns
GalNAcEXO® Immobilized Microspin 10 x 0.5 mg
Immobilized enzyme for hydrolysis of GalNAc residues on 10 x 0.5 mg glycoprotein in spin columns
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
0.1 ml of settled GalNAcEXO Immobilized agarose resin catalyzes the hydrolysis of α-linked GalNAc residues from ≥ 95% of 5.5 µmol 4-Nitrophenyl 2-acetamido-2-deoxy-a-D-galactopyranoside when incubated in 20 mM Tris, 10 % ethanol pH 6.8 for 5 minutes at room temperature.
One Microspin column contains sufficient GalNAcEXO-coupled agarose beads to hydrolyze α-linked GalNAc residues on 0.5 mg glycoprotein.
Content and Storage
GalNAcEXO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
O-glycan site occupancy profiling and complete deglycosylation of etanercept for in-depth characterization of complex O-glycoproteins.
FAQ and Support
Does GalactEXO digest fucosylated glycan structures such as Lewis X or Lewis a?
No. Branching fucose linked to the same residue as the β-galactose inhibits GalactEXO activity and needs to be removed prior to GalactEXO treatment. The FucosEXO enzyme can be used for hydrolysis of α1-2, α1-3 and α1-4 linked fucose.
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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