Description
Immobilized GalNAcEXO is a resin with an α-N-Acetylgalactosaminidase covalently coupled to agarose beads in microspin column format for convenient and efficient hydrolysis of α-N-acetylgalactosamine (GalNAc) linked to serine or threonine residues on glycoproteins (Tn antigen). The enzyme also displays activity on α1-3 linked terminal GalNAcs. Immobilized GalNAcEXO hydrolyzes GalNAc on glycoproteins under native conditions and is highly active in the pH range 6.0 to 7.6. No cofactors or special buffers are required. The enzyme in Immobilized GalNAcEXO is derived from Akkermansia muciniphila, expressed in E. coli with a His-tag, and has a molecular weight of 52 kDa.