Powered by BiozHydrolysis of Sialic Acids
The SialEXO products are sialidases for the removal and analysis of sialic acids. The enzymes are active on both N- and O-linked glycans present on native glycoproteins or released glycan structures.
SialEXO is used to pretreat O-glycosylated proteins prior to digestion with OpeRATOR or OglyZOR. Other applications include reduction of sample complexity, charge variant analyses and exoglycosidase arrays.
- Efficient desialylation of N-linked, O-linked and released glycans
- Fast removal of sialic acids for enhanced analysis of protein charge variants
- Available immobilized in ready-to-use spin columns
Download product folderSialEXO® Lyophilized
Lyophilized enzyme mix for desialylation of glycoproteinsSialEXO® 2-3 Lyophilized
Lyophilized enzyme for hydrolysis of ɑ2-3-linked sialic acidsSialEXO® Immobilized
Immobilized enzymes for desialylation of glycoproteins in spin columnsAbout SialEXO®
The SialEXO products are sialidases for efficient desialylation of N- and O-glycosylated proteins.
SialEXO Lyophilized consists of two sialidases, each having unique activities and they work simultaneously. One enzyme has a broad activity for α2-3, α2-6 and α2-8-linked sialic acids, and the other one (SialEXO 2-3) quickly hydrolyzes sialic acids with α2-3-linkage. SialEXO Lyophilized removes all sialic acids on native proteins within 2 h.
SialEXO hydrolyzes glycoproteins under native conditions and displays activity in pH ranging from 6.5 to 9.
The enzymes are derived from Akkermansia muciniphila and expressed in E. coli. Both enzymes contain a His-tag and the molecular weights of the enzymes are 43 kDa and 66 kDa.
SialEXO® Citations
SialEXO® Lyophilized
Lyophilized enzyme mix for desialylation of glycoprotein.
SialEXO Lyophilized is available as a lyophilized powder in 2000 unit vials for desialylation of 2 mg glycoprotein.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Complete Desialylation of Biopharmaceuticals
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Complete N- and O-Deglycosylation of Biopharmaceuticals
PEGS Europe, 2019
Unit Definition
One unit of SialEXO Lyophilized hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.
Content and Storage
SialEXO Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
SialEXO® 2-3 Lyophilized
Lyophilized enzyme for hydrolysis of ɑ2-3-linked sialic acids.
SialEXO 2-3 Lyophilized is available as a lyophilized powder in 500 unit vials for desialylation of 0.5 mg glycoprotein.
In contrast to SialEXO, which is a sialidase mix, SialEXO 2-3 is an α2-3 specific sialidase, allowing targeted analysis of α2-3 linked sialic acids only. Efficient α2-3 desialylation of O- and N-glycosylated proteins can be achieved within 1 h.
SialEXO 2-3 hydrolyzes glycoproteins under native conditions and displays activity in pH ranging from 7.0 to 9.0.
The enzyme is derived from Akkermansia muciniphila and expressed in E. coli. The enzyme has a His-tag and the molecular weight is 66 kDa.
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Unit Definition
One unit of SialEXO 2-3 Lyophilized hydrolyzes α2-3 linked sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 7.5 at 37 °C for 1 h.
Content and Storage
SialEXO 2-3 Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
SialEXO® Immobilized
Immobilized enzymes for desialylation of glycoproteins in spin columns.
The SialEXO Immobilized spin columns contain SialEXO enzyme covalently coupled to agarose beads, for desialylation of glycoproteins without contaminating the final preparation with enzyme. It hydrolyzes sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins and released glycans.
The Microspin columns are available for desialylation of 2 x 0.5 mg, 5 x 0.5 mg, or 10 x 0.5 mg glycoprotein.
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Unmasking Charge Variants
Unit Definition
0.1 ml of settled SialEXO Immobilized agarose resin hydrolyzes sialic acids from > 90% of 0.5 mg C1 inhibitor in 30 min at room temperature in PBS pH 7.4 as monitored by ion exchange chromatography.
One Microspin column contains sufficient SialEXO-coupled agarose beads to desialylate 0.5 mg glycoprotein.
Content and Storage
SialEXO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Yes, SialEXO contains a 6x His-tag.
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
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