The Digested Guide to ASMS 2018

May 29, 2018 | Applications, Conferences | Maria Ekemohn

Several researchers have submitted abstracts for ASMS 2018 in San Diego, in which Genovis’ SmartEnzymes have been used. Below is a selection of these abstracts.

To read the poster abstracts, visit the Online Planner for ASMS, and paste the abstract titel in the search field.

Monday June 4

10:30AM-2:30PM: Poster Session

MP 045 – GlyCLICK

Development of NISTmAb-derived homogeneous antibody-drug conjugate (ADC) standards

Shanhua Lin¹; Terry Zhang²; Brian Agnew³; Trina Mouchahoir⁴; John Schiel⁴¹Thermo Fisher Scientific, Sunnyvale, CA; ²Thermo Fisher Scientific, San Jose, CA; ³Thermo Fisher Scientific, Eugene, Oregon; ⁴NIST, Gaithersburg, MD

MP 046 – FabRICATOR

Discovery and confirmation of glucuronylation as a new acidic post-translational modification on therapeutic monoclonal antibodies

Yuetian Yan¹; Anita Liu¹; Shunhai Wang¹; Thomas Daly¹; Ning Li¹¹Regeneron Pharmaceuticals, Tarrytown, NY

MP 049 – FabRICATOR

Ultrasensitive Characterization of Size and Charge Heterogeneity of Therapeutic Monoclonal Antibodies by Native Mass Spectrometry

Shunhai Wang¹; Yuetian Yan¹; Anita Liu¹; Thomas Daly¹; Ning Li¹¹Regeneron Pharmaceuticals, Tarrytown, NY

MP 464 – FabALACTICA

Quantitative UPLC-MS^E analysis of disulfide bonds and free sulfhydryls in monoclonal antibodies using IgdE protease assisted digestion

Jeroen de Keijzer¹; Peter van Maurik¹; Anja Boumeester¹; Emile van Corven¹; Gideon Oudgenoeg¹¹Bioceros, Utrecht, Netherlands

Tuesday June 5

10:30AM-2:30PM: Poster Session

TP 624 – FabRICATOR

Avoiding method induced heterogeneity in the analysis of heterogeneity of monoclonal antibodies using Mass Spectrometry after Single Site Proteolysis

Gideon Oudgenoeg¹; Anja Boumeester²; Peter van Maurik²; Jeroen de Keijzer²; Emile van Corven²¹Bioceros, Utrecht, Netherlands; ²Bioceros, Utrecht, Netherlands

Wednesday June 6

10:30AM-2:30PM: Poster Session

WP056 – FabRICATOR

Characterizing and Quantitating Therapeutic Antibody Multimer Degradation Using Affinity Capture Mass

Neha Srikumar¹; Wenjing Li¹; Robert Tchelepi¹; Chen Gu¹; Diego Ellerman¹; Greg A Lazar¹; Yichin Liu¹; John C. Tran¹¹Genentech Inc., South San Francisco, CA

WP 327 – GingisKHAN

Comprehensive Domain-Specific [Fc vs. Fab] N-glycosylation Analysis of Therapeutic Proteins

Charles Nwosu¹; Shuangqi Sally Liu²; Lei Wang²; May Zhu²; Anne Kowal²¹Takeda Pharmaceuticals International Co, Cambridge, MA; ²Takeda Pharmaceuticals International Co., Cambridge, MA

WP 340 – FabULOUS

Analysis of Fragmented Porcine Immunoglobulin G (IgG) by MALDI-MS and UPLC-ESI-MS

HELENE PERREAULT¹; Claudia Nelson²¹University of manitoba, Winnipeg; ²University of Manitoba, Winnipeg, MB

WP 342 – OpeRATOR and GlycOCATCH

A Novel O-glycoprotease with applications in O-glycan Analysis using mass spectrometry

Rolf Lood^{1, 2}; Maria Nordgren¹; Fredrik Leo¹; Stephan Björk¹; Malin Mejáre¹; Fredrik Olsson¹¹Genovis AB, Lund, Sweden; ²Department of Infectious Diseases, Lund University, Lund, Sweden

WP 350 – OpeRATOR

Deciphering complex o-glycosylation: solid-phase chemoenzymatic cleavage and enrichment

Shuang Yang¹; Philip Onigman²; Jonathan Sjogren²; Wells W. Wu³; Rong-fong Shen³; John Cipollo¹¹LBP, CBER, FDA, Silver Spring, MD; ²Genovis AB Inc., Boston, MA; ³FBR, CBER, FDA, Silver Spring, MD

WP 676 –FabRICATOR, GlycINATOR, IgGZERO

Monitoring Critical Quality Attributes: Core Fucosylation of N-glycans using an Integrated Subunit LC/MS Workflow Method

Nilini S Ranbaduge¹; Henry Y Shion¹; Ying Qing Yu¹; Weibin Chen¹¹Waters Corporation, Milford, MA

WP 678 – FabRICATOR

In-depth Characterization of the Heterogeneous Dimerization Interfaces of A Monoclonal Antibody: from Subdomain Level to Residue Level

Yuetian Yan¹; Shunhai Wang¹; Thomas Daly¹; Ning Li¹¹Regeneron Pharmaceuticals, Tarrytown, NY

W 691 – OpeRATOR, OglyZOR and SialEXO

LC-MS Characterization of Complex Glycoproteins

Amber Peariso¹; Jason X. Tang¹¹Eli Lilly & Company, Indianapolis, IN

WP 692 – FabRICATOR

Rapid Identity Assays for mAb Development, Production Control and Release

Anja Resemann¹; Waltraud Evers¹; Yue Ju²; Guillaume Tremintin²; Detlev Suckau¹¹Bruker Daltonics, Bremen, Germany; ²Bruker Daltonics, Billerica, MA

Thursday June 7

10:10AM-10:30PM: Oral Presentation

Oral Presentation – FabRICATOR

Classification of Plasma Cell Disorders by 21 Tesla FT-ICR Top-Down and Middle-Down MS/MS Analysis of Monoclonal Immunoglobulins in Human Serum

Lidong He^{1, 2}; Lissa C Anderson²; David R Barnidge³; David L Murray⁴; Surendra Dasari⁴; Angela Dispenzieri⁴; Christopher L Hendrickson^{1, 2}; Alan G Marshall^{1, 2}¹Florida State University, Tallahassee, FL; ²National High Magnetic Field Laboratory, Tallahassee, FL; ³The Binding Site, Rochester, MN; ⁴Mayo Clinic, Rochester, MN

10:30AM-2:30PM: Poster Session

ThP 003 – FabRICATOR

LC-MS in Combination with Multiple Enzymatic Digestion for Sequence Variant Identification in Support of Cell Line Development

Renpeng Liu¹; Lintao Wang¹; Alexandru C. Lazar¹¹ImmunoGen, Waltham, MA

ThP 017 – GingisKHAN

Consortium for Top-Down Proteomics Inter-laboratory Study for Characterizing Monoclonal Antibodies (mAbs) by Top-Down Mass Spectrometry

Kristina Srzentic¹; Luca Fornelli¹; Yury Tsybin²; Joseph Loo³; Jeffrey Agar⁴; Julia Chamot-Rooke⁵; Paul Danis⁶; Ying Ge⁷; David Goodlett⁸; Neil Kelleher¹; Ljiljana Pasa Tolic⁹; Lloyd Smith⁷; Timothy Toby¹; Konstantin Nagornov²; JENNIFER BRODBELT¹⁰; Sylvester Greer¹⁰; Mathieu Dupré⁵; David Clarke¹¹; Ziqing Lin⁷; Kim Haselmann¹²; Christopher Hendrickson¹³; Lidong He¹³; Donald Hunt¹⁴; Jared Shaw⁹; Wendy Sandoval¹⁵; Richa Sarin¹⁶; Detlev Suckau¹⁷; Yuri E.M. van der Burgt¹⁸; Norelle Wildburger¹⁹; Nicolas L. Young²⁰; Alain Beck²¹; John Yates²²; Jolene Diedric²²; Sneha Chatterjee²³; Frank Sobott²⁴; Anton Kozhinov²; ALAN G. MARSHALL¹³; LISSA C. ANDERSON¹³; Natalia Gasilova²⁵; Laure Menin²⁵; Neil Quebbenamm³; Sung Hwan Yoon²⁶; Josh Hinkle¹⁴; Simone Nicolardi¹⁸; Matthew V. Holt²⁰; Yunqiu Chen¹⁶; Nicholas Schmitt⁴¹Northwestern University, Evanston, IL; ²Spectroswiss Sàrl, Lausanne, Switzerland; ³UCLA, Los Angeles, CA; ⁴Northeastern University, Boston, MA; ⁵Institute Pasteur, Paris, France; ⁶Eastwoods Consulting, Boylston, MA; ⁷University of Wisconsin, Madison, WI; ⁸University of Maryland, Baltimore, Baltimore; ⁹PNNL, Richland, WA; ¹⁰University of Texas at Austin, Austin, TX; ¹¹Edinburgh University, Edinburgh, United Kingdom; ¹²Novo Nordisk, Malov, Denmark; ¹³National High Magnetic Field Laboratory, Tallahassee, FL; ¹⁴University of Virginia, Charlottesville, VA; ¹⁵Genentech, Inc., South San Francisco, CA; ¹⁶Biogen Inc, Cambridge, MA; ¹⁷Bruker Daltonik GmbH, Bremen, Germany; ¹⁸Leiden University Medical Centre, Leiden, Netherlands; ¹⁹Washington University, St. Louis, St. Louis, MO; ²⁰Baylor College of Medicine, Houston, TX; ²¹Centre d’immunologie Pierre Fabre, Saint-Julien-en-Genevois, France; ²²The Scripps Research Institute, La Jolla, CA; ²³University of Antwerp, Antwerp, Belgium; ²⁴University of Leeds, Leeds, United Kingdom; ²⁵Ecole Polytechnique Fédérale de Lausanne, Ch-1015 Lausanne, Switzerland; ²⁶University of Maryland, Baltimore, MD

ThP 028 – FabRICATOR

Top- and Middle-Down CE-ESI-MS Analysis of Intact mAbs Using the ZipChip Coupled to a Fusion Lumos ETD Mass Spectrometer

Tricia C. Ho¹; Erik J. Soderblom¹; Erin Redman²; Greg M. Waitt¹; M. Arthur Moseley¹¹Duke University School of Medicine, Proteomics and Metabolomics Shared Resource, Durham, NC; ²908 Devices, Inc., Carrboro, NC

ThP 419 – FabRICATOR

Quantitation of Misincorporations: Strategies and System Suitability

Kathleen Cornelius¹; Olga Friese¹; Mary Denton²; Jason Rouse²¹Pfizer, Inc, Chesterfield, MO; ²Pfizer Inc., Andover, MA

2:30 -2:50 PM: Oral Presentation

Ballroom 20D – FabRICATOR, GingisKHAN, GlycINATOR and IgGZERO

A Suite of Liquid Chromatography Strategies Coupled Online to Top-down High-resolution Mass Spectrometry for Comprehensive Analysis of Antibody Drug Conjugates

Bifan Chen¹; Ziqing Lin¹; Qingge Xu¹; Cexiong Fu²; Qunying Zhang²; Ying Ge¹¹University of Wisconsin-Madison, Madison, Wisconsin; ²Abbvie Inc., North Chicago, IL