GlyCLICK SmartEnzymes™ logo

glycklick-flow visualization

 

GlyCLICK®

GlyCLICK is a site-specific conjugation technology for IgG using Fc glycan remodeling and click-chemistry.

The technology generates stable and homogenous antibody conjugates for IgG from several species and subclasses. Fc-glycan remodeling by complete deglycosylation of the antibody allows for site-specific conjugation using robust click-chemistry, resulting in a degree of label (DOL) or antibody-drug ratio (DAR) of 2.0.

The reliable performance ensures quantitative conjugation and intact immunoreactivity for sensitive applications.  The technology is available in a range of kit formats to facilitate tailored conjugates by site-specific labeling of antibodies using customized labels.


Alexa Fluor 488

In vitro cell imaging, IHC, FCM
250μg (L1-F01-025) 925.00
2mg (L1-F01-200) 1,570.00

Alexa Fluor 555

In vitro cell imaging, IHC, FCM
250μg (L1-F02-025) 925.00
2mg (L1-F02-200) 1,710.00

Alexa Fluor 647

In vitro cell imaging, IHC, FCM
250μg (L1-F03-025) 925.00
2mg (L1-F03-200) 1,710.00

Biotin

Immunoassays, ELISA, WB
250μg (L1-A01-025) 925.00
2mg (L1-A01-200) 1,570.00

DFO

Immuno- and In vivo Imaging
250μg (L1-C01-025) 925.00
2mg (L1-C01-200) 1,570.00

ADC MMAE

ADC Development
2mg (L1-T02-200) 1,750.00

ADC PNU

ADC Development
2mg (L1-T01-200) 1,990.00

Azide Activation

Custom Conjugation
250μg (L1-AZ1-025) 825.00
2mg (L1-AZ1-200) 1,310.00
10mg (L1-AZ1-100) 5,250.00

GlyCLICK workflow visualization
Figure 1.Schematic presentation of the GlyCLICK conjugation process.

How GlyCLICK works

  1. Deglycosylation
    The Fc specific Endoglycosidase GlycINATOR® (EndoS2)1 hydrolyzes the Fc glycans to the inner most GlcNAc moiety on several subclasses and species of IgG. The enzyme removes all glycoforms, including; high-mannose, hybrid, complex, and bisecting type glycans.
  2. Azide Activation
    Azide-containing UPD-GalNAz is enzymatically attached to the exposed GlcNAc using the ß-1,4-Galactosyltransferase Y289L* (GalT) to generate an azide-activated antibody that is reactive with an alkyne-carrying label.
  3. CLICK Reaction
    The azide activated antibody is conjugated with a functionalized label2 of choice in a biorthogonal reaction through strain-promoted copper-free click chemistry (SPAAC)3 to form a stable triazole.

Characteristics

GlyCLICK enables site-specific and quantitative conjugation of IgG from several species and subclasses with a degree of label (DOL) of 2. The specificity of GlycINATOR allows for the complete deglycosylation of all IgG Fc-glycoforms and does not require any prior engineering of the amino acid sequence. Robust conjugation using click-chemistry results in stable and completely labeled antibody conjugates with minimized the variation between batches.

 

  • Human IgG1-4, Fc-fusion proteins, IgG from mouse, rabbit, rat, monkey, sheep, goat, cow and horse
  • ~2-3 day protocol
  • Available labels: AlexaFluor®488, biotin and DFO. Azide activation kits are available for custom conjugation

Applications

GlyCLICK contains

  • Immobilized GlycINATOR
  • UDP-GalNAz
  • GalT(Y298L)
  • sDIBO label of choice
  • All buffers needed
  • Concentration and desalting columns

Unit definition

GlyCLICK contains material sufficient for azide activation or labeling of 250 μg, 2 mg or 10 mg of antibody. For larger amounts or evaluation of the technology, please contact us using the contact form below.

Place an Order

For details on ordering please visit How to order. Click add to cart
above to buy GlyCLICK online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

Download GlyCLICK Posters

Genvois posters on characteristics and applications for the GlyCLICK technology.

All GlyCLICK References

All GlyCLICK References

Published research articles on GlyCLICK and applications for the technology.

Download GlyCLICK Brochure

Download GlyCLICK Brochure

GlyCLICK is a site-specific conjugation technology using enzymatic remodeling and click-chemistry.

Contact

For custom made formats or licensing opportunities of GlyCLICK, please contact the Genovis team.

By providing us with your contact details, you accept our Data Protection Policy.

Required field

References

1. Sjögren, J. et al., 2013. EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and a1-acid glycoprotein. The Biochemical Journal, 455(1), pp.107–118.
2. SiteClick™ is provided under an intellectual property license from Life Technologies Corporation. The trademark SiteClick™ is the property of Life Technologies Corporation.
3. Ramakrishnan, B. & Qasba, P.K., 2002. Structure-based design of beta 1,4-galactosyltransferase I (beta 4Gal-T1) with equally efficient N-acetylgalactosaminyltransferase activity: point mutation broadens beta 4Gal-T1 donor specificity. J Biol Chem, 277(23), pp.20833–20839.

Order