GlyCLICK

glycklick-flow  

GlyCLICK™

GlyCLICK is a site-specific conjugation technology for antibodies based on enzymatic remodeling of the Fc glycans and click chemistry. The technology covalently conjugates a label of choice to the Fc domain of the antibody with a degree of label of 2, resulting in a homogenous antibody conjugate with preserved immunoreactivity.

GlyCLICK is available with the following DIBO conjugates: Alexa Fluor® 488, biotin and deferoxamine (DFO). The kit is also available with azide activation-only, for custom conjugation with any alkyne label.

Product Description

GlyCLICK is a unique conjugation technology for site-specific labeling of antibodies at the N-linked glycans in the Fc domain. The IgG-specific endoglycosidase GlycINATOR (EndoS2) is utilized for its ability to hydrolyze IgG Fc glycans after the core GlcNAc1. Using an engineered galactosyl transferase, GalT(Y289L), a GalNAz residue is subsequently attached to the exposed GlcNAc, resulting in an azide available for a biocompatible strain-promoted azide-alkyne chemistry reaction2 with a dibenzocyclooctyne (DIBO)-modified label3.

A wide variety of DIBO-modified labels (functional groups) can be specifically conjugated to the antibody including drugs, chelators, biotin or fluorophores. Using GlyCLICK, functional groups can be conjugated to the Fc domain of antibodies of several species and a variety of subclasses. The technology results in a ratio of 2 conjugates per antibody with high reproducibility and a low batch-to-batch variation. GlyCLICK is applicable to all IgG antibodies carrying Fc glycans and does not rely on prior engineering of the amino acid sequence.

The applications for the GlyCLICK-technology includes e.g. cytotoxic drug development, in vitro and in vivo cell imaging, immunohistochemistry and western blotting.


(1) Sjögren, J. et al., 2013. EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and α1-acid glycoprotein. The Biochemical Journal, 455(1), pp.107–118.

(2) SiteClick™ is provided under an intellectual property license from Life Technologies Corporation. The trademark SiteClick™ is the property of Life Technologies Corporation.

(3) Ramakrishnan, B. & Qasba, P.K., 2002. Structure-based design of beta 1,4-galactosyltransferase I (beta 4Gal-T1) with equally efficient N-acetylgalactosaminyltransferase activity: point mutation broadens beta 4Gal-T1 donor specificity. J Biol Chem, 277(23), pp.20833–20839.

Key Characteristics

  • Site-specific conjugation
  • Degree of label (DOL) = 2
  • Intact immunoreactivity
  • Robust performance
  • Modular technology

Applications

  • ADC Development
    Conjugate drugs to the Fc portion of an antibody using GlyCLICK
  • In vivo imaging
    An antibody carrying a DFO chelator allows in vivo imaging

All GlyCLICK Applications

Download GlyCLICK Poster

2017_ATDC_Site-specific and Quantitative Conjugation of Functional Groups to Antibody N-glycans using the Novel GlyCLICKTM Technology

Unit Definition

GlyCLICK contains 250 units for conjugation of 250 μg of antibody. For larger amounts or for evaluation of GlyCLICK for ADC projects, please contact us using the contact form below.

GlyCLICK Contains

  • Immobilized GlycINATOR
  • UDP-GalNAz
  • GalT(Y298L)
  • DIBO-modified label of choice
  • All buffers needed
  • Concentration and desalting columns

How to order

For details on ordering please visit How to order. Click add to cart above to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

How GlyCLICK works

IgG contain a conserved N-linked glycosylation site on the CH2 domain of each heavy chain of the Fc region. This site of the antibody is used by GlyCLICK for selective conjugation of a label of choice. The labeling is performed in three steps:

  1. Immobilized GlycINATOR (EndoS2) hydrolyzes the N-glycans on the Fc domain of the IgG, leaving the innermost GlcNac residue intact.
  2. Specific attachment of a GalNAz residue to the core GlcNAc by an engineered galactose transferase (GalT(Y298L)).
  3. The azide-activated antibody reacts with a DIBO-alkyne label in a strain-promoted, copper-free click chemistry reaction and forms a stable and homogenous antibody conjugate.

GlyCLICK_Detaljerad_Flow_Arbetsfil-02

Modular Technology

Highly versatile method for conjugation of a variety of labels to IgG.

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Reliable Performance

Robust method that can be used to modify IgGs of many species and subclasses.

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Precise and complete conjugation

Site specific conjugation on the Fc domain preserving the antigen binding activity.

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Documents

Contact

For custom made formats or licensing opportunities of GlyCLICK, please fill out the form below.

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Patent and Disclaimer

GlyCLICK™

 

GlycINATOR®

All rights reserved. Aspects of GlycINATOR technology are encompassed by pending patent applications and by granted Patent in Singapore, China, USA and EPO in the name of Genovis AB. The trademark GlycINATOR is the property of Genovis AB.

The trademark GlyCLICK is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.

 

SiteClick™

This product is provided under an intellectual property license from Life Technologies Corporation.  The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or  (2) sell or transfer this product or its components for resale, whether or not resold for use in research.  For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

The trademark SiteClick™ is the property of Life Technologies Corporation.

©2017 Genovis AB.