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glycklick-flow visualization

 

GlyCLICK®

GlyCLICK is a site-specific conjugation technology for IgG using Fc glycan remodeling and click-chemistry.

The technology generates stable and homogenous antibody conjugates for IgG from several species and subclasses. Fc-glycan remodeling by complete deglycosylation of the antibody allows for site-specific conjugation using robust click-chemistry, resulting in a degree of label (DOL) or antibody-drug ratio (DAR) of 2.0.

The reliable performance ensures quantitative conjugation and intact immunoreactivity for sensitive applications.  The technology is available in a range of kit formats to facilitate tailored conjugates by site-specific labeling of antibodies using customized labels.


Alexa Fluor 488

In vitro cell imaging, IHC, FCM
250μg (L1-F01-025) 925.00
2mg (L1-F01-200) 1,495.00

Alexa Fluor 555

In vitro cell imaging, IHC, FCM
250μg (L1-F02-025) 925.00
2mg (L1-F02-200) 1,625.00

Alexa Fluor 647

In vitro cell imaging, IHC, FCM
250μg (L1-F03-025) 925.00
2mg (L1-F03-200) 1,625.00

Biotin

Immunoassays, ELISA, WB
250μg (L1-A01-025) 925.00
2mg (L1-A01-200) 1,495.00

DFO

Immuno- and In vivo Imaging
250μg (L1-C01-025) 925.00
2mg (L1-C01-200) 1,495.00

Azide Activation

Custom Conjugation
250μg (L1-AZ1-025) 825.00
2mg (L1-AZ1-200) 1,250.00
10mg (L1-AZ1-100) 5,145.00

How GlyCLICK works

GlyCLICK workflow visualization
Figure 1.Schematic presentation of the GlyCLICK conjugation process.

  1. Deglycosylation
    The Fc specific Endoglycosidase GlycINATOR® (EndoS2)1 hydrolyzes the Fc glycans to the inner most GlcNAc moiety on several subclasses and species of IgG. The enzyme removes all glycoforms, including; high-mannose, hybrid, complex, and bisecting type glycans.
  2. Azide Activation
    Azide-containing UPD-GalNAz is enzymatically attached to the exposed GlcNAc using the ß-1,4-Galactosyltransferase Y289L* (GalT) to generate an azide-activated antibody that is reactive with an alkyne-carrying label.
  3. CLICK Reaction
    The azide activated antibody is conjugated with a functionalized label2 of choice in a biorthogonal reaction through strain-promoted copper-free click chemistry (SPAAC)3 to form a stable triazole.

Characteristics

GlyCLICK enables site-specific and quantitative conjugation of IgG from several species and subclasses with a degree of label (DOL) of 2. The specificity of GlycINATOR allows for the complete deglycosylation of all IgG Fc-glycoforms and does not require any prior engineering of the amino acid sequence. Robust conjugation using click-chemistry results in stable and completely labeled antibody conjugates with minimized the variation between batches.

 

Key Characteristics

  • Human IgG1-4, Fc-fusion proteins, IgG from mouse, rabbit, rat, monkey, sheep, goat, cow and horse
  • ~2-3 day protocol
  • Available labels: AlexaFluor®488, biotin and DFO. Azide activation kits are available for custom conjugation

SmartStories

Dynamic fluorescence imaging using site-specific antibody conjugates

Applications

The unique combination of enzymes in GlyCLICK generates robust and quantitatively labeled antibody conjugates. The ability to conjugate various species and subclasses of IgG using a functionalized label of choice enables a wide range of applications including fluorescent and in vivo imaging, immunoassays and ADC development.

Fluorescent Conjugates

Fluorescent Conjugates

 
Site-specific antibody conjugates using various fluorescent dyes for a wide range of imaging applications.

Custom Conjugates

 
Tailored conjugates using a custom label or payload for homogenous and robust antibody conjugates.

Radiolabeled Conjugates

 
Quantitative conjugation for high quality chelation and radio-labeling of antibodies for in vivo imaging.

Place an Order

For details on ordering please visit How to order. Click add to cart
above to buy GlyCLICK online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

GlyCLICK contains

  • Immobilized GlycINATOR
  • UDP-GalNAz
  • GalT(Y298L)
  • sDIBO label of choice
  • All buffers needed
  • Concentration and desalting columns
GlyCLICK characters

Unit definition

GlyCLICK contains material sufficient for azide activation or labeling of 250 μg, 2 mg or 10 mg of antibody. For larger amounts or evaluation of the technology, please contact us using the contact form below.
 

 

Download Posters and References

Download GlyCLICK Posters

Genvois posters on characteristics and applications for the GlyCLICK technology.

All GlyCLICK References

All GlyCLICK References

Published research articles on GlyCLICK and applications for the technology.

Download GlyCLICK Brochure

Download GlyCLICK Brochure

GlyCLICK is a site-specific conjugation technology for IgG using enzymatic remodeling and click-chemistry.

Documents

Contact

For custom made formats or licensing opportunities of GlyCLICK, please fill out the form below.


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Legal and Disclaimers

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals

 

SiteClick™ included in GlyCLICK®

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

©2019 Genovis AB.

 

1. Sjögren, J. et al., 2013. EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and a1-acid glycoprotein. The Biochemical Journal, 455(1), pp.107–118.
2. SiteClick™ is provided under an intellectual property license from Life Technologies Corporation. The trademark SiteClick™ is the property of Life Technologies Corporation.
3. Ramakrishnan, B. & Qasba, P.K., 2002. Structure-based design of beta 1,4-galactosyltransferase I (beta 4Gal-T1) with equally efficient N-acetylgalactosaminyltransferase activity: point mutation broadens beta 4Gal-T1 donor specificity. J Biol Chem, 277(23), pp.20833–20839.

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