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FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at one specific site above the hinge with no need for reducing conditions or co-factors.


FabALACTICA is available in three different product formats:

Product Information

FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge (KSCDKT / HTCPPC), generating intact Fab and Fc fragments. The name of the enzyme is derived from the pathogen Streptococcus agalactiae, where it was first discovered (Spoerry et al., 2016). The enzyme is active at pH from 6-8 and does not require reducing conditions or co-factors for activity.

FabALACTICA is recombinantly expressed in E. coli, contains a His-tag, and the molecular weight is 70 019 Da.

Spoerry, C. et al., 2016. Novel IgG-Degrading Enzymes of the IgdE Protease Family Link Substrate Specificity to Host Tropism of Streptococcus Species. PloS one, 11(10), p.e0164809.

Key Characteristics

  • Human IgG1
  • Overnight (O/N) reaction
  • No need for reducing agents or co-factors
  • KSCDKT / HTCPPCP (above the hinge)


  • Subunit LC/MS analysis
  • Paired glycan analysis
  • Characterization of bi- or multi specific antibodies
  • Affinity screening
  • Studies of higher order structures
  • Studies on antibodies with mutated hinges

Product Formats


FabALACTICA enzyme supplied as lyophilized powder. The enzyme is available in 2000 unit vials for digestion of up to 2 mg human IgG1.

Immobilized FabALACTICA®

FabALACTICA enzyme immobilized on agarose, allowing for human IgG1 digestion without contaminating the final preparation with FabALACTICA enzyme.

FabALACTICA® Fab kit

FabALACTICA Fab kit is used for easy preparation and purification of Fab fragments from human IgG1. The kit consists of two parts; Immobilized FabALACTICA and affinity spin columns for purification of intact Fab fragments.

FabALACTICA Brochures, Posters and Applications

Download FabALACTICA igde Posters

Brochures and Posters

Download Genovis brochures and posters with the latest details on the SmartEnzymes


FabALACTICA in non-reducing study of antibody disulphides

FabALACTICA igde Applications


Learn more about the applications of Genovis SmartEnzymes

Download FabALACTICA Posters and External References

FabALACTICA igde poster 1

Poster from FoB 2019

"FabALACTICA Generates Pure and Homogeneous mAb Subunits that Facilitate 2D-NMR Spectroscopy Analysis."


"In this application note, an analytical workflow for studying intact Fc fragments using an above hinge digestion with FabALACTICA is used for paired glycan analysis."

FabALACTICA references


Find external articles and papers using FabALACTICA





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