FabALACTICA

FabALACTICA

FabALACTICA® (IgdE)

FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at one specific site above the hinge with no need for reducing conditions or co-factors.

 

FabALACTICA is available in three different product formats:

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Product Information

FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge (KSCDKT / HTCPPC), generating intact Fab and Fc fragments. The name of the enzyme is derived from the pathogen Streptococcus agalactiae, where it was first discovered (Spoerry et al., 2016). The enzyme is active at pH from 6-8 and does not require reducing conditions or co-factors for activity.

FabALACTICA is recombinantly expressed in E. coli, contains a His-tag, and the molecular weight is 70 019 Da.

 


Spoerry, C. et al., 2016. Novel IgG-Degrading Enzymes of the IgdE Protease Family Link Substrate Specificity to Host Tropism of Streptococcus Species. PloS one, 11(10), p.e0164809.

Key Characteristics

  • Human IgG1
  • Overnight (O/N) reaction
  • No need for reducing agents or co-factors
  • KSCDKT / HTCPPCP (above the hinge)

Applications

  • Subunit LC/MS analysis
  • Paired glycan analysis
  • Characterization of bi- or multi specific antibodies
  • Affinity screening
  • Studies of higher order structures
  • Studies on antibodies with mutated hinges

Product Formats

FabALACTICA® Enzyme

FabALACTICA enzyme supplied as lyophilized powder. The enzyme is available in 2000 unit vials for digestion of up to 2 mg human IgG1.

Immobilized FabALACTICA®

FabALACTICA enzyme immobilized on agarose, allowing for human IgG1 digestion without contaminating the final preparation with FabALACTICA enzyme.

FabALACTICA® Fab kit

FabALACTICA Fab kit is used for easy preparation and purification of Fab fragments from human IgG1. The kit consists of two parts; Immobilized FabALACTICA and affinity spin columns for purification of intact Fab fragments.

Latest References using this Product

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Applications

Applications

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Antibody Characterization

WCBP 2018

"Enzymatic Strategies for Generation of Intact Fab and Fc Fragments - IgdE Results in Strict Specific Digestion Compared to Lys-C and Papain"

Antibody Characterization

VIB 2017

"Preparation of Pure Antibody Fab Fragments Using a Robust and Specific Enzyme (IgdE)"

Antibody Characterization

CASSS 2017

"Characterization of a Novel Protease (IgdE) for Above Hinge Antibody Subunit Generation"

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Legal and Disclaimers

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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.