FabALACTICA™
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FabALACTICA™ Immobilized
Immobilized enzyme for above hinge digestion of human IgG1 in spin columns
FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact and homogenous Fab and Fc fragments.
The FabALACTICA Immobilized spin columns contain FabALACTICA enzyme covalently coupled to agarose beads, for digestion of human IgG1 without contaminating the final preparation with enzyme.
The spin columns are available for digestion of up to 0.5 mg (Microspin), 10 mg (Midispin) or 100 mg (Maxispin) human IgG1. The smallest columns are available in two different pack sizes, with 2 or 10 columns per pack.
Why FabALACTICA™ Immobilized?
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
FabALACTICA™ Immobilized Microspin 2 x 0.5 mg
Immobilized enzyme for above hinge digestion of 2 x 0.5 mg human IgG1 in spin columns
FabALACTICA™ Immobilized Microspin 10 x 0.5 mg
Immobilized enzyme for above hinge digestion of 10 x 0.5 mg human IgG1 in spin columns
FabALACTICA™ Immobilized Midispin 5-10 mg
Immobilized enzyme for above hinge digestion of 5-10 mg human IgG1 in spin columns
FabALACTICA™ Immobilized Maxispin 50-100 mg
Immobilized enzyme for above hinge digestion of 50-100 mg human IgG1 in spin columns
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
Content and Storage
FabALACTICA Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Performance Activity
Simple preparation of highly pure Fab fragments from IgG1 antibodies using FabALACTICA digestion combined with Fc affinity purification.
Citations
Powered by BiozFAQ and Support
Can I digest Fc-fusion proteins using FabALACTICA (IgdE)?
No, FabALACTICA has shown very poor activity on Fc-fusion proteins.
We want to make Fab’s directly from antibodies. When would the selection of FabALACTICA be the first choice over GingisKHAN or FabULOUS?
FabALACTICA does not require reducing conditions as both GingisKHAN and FabULOUS does, and allows for generation of Fab fragments from native human IgG1.
Can I release Fc fragments from the CaptureSelect column after isolating the Fab fragments?
It is not recommended to elute the Fc fragments from the CaptureSelect column since the fraction might also include undigested or semi digested material.
Can I re-use FabALACTICA Immobilized columns?
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
What is the difference between FabALACTICA and GingisKHAN?
FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (…KSCDKT / HTCPPCP…) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (…KSCDK/THTCPPCP…).
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