FabALACTICA™
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FabALACTICA™ Fab Kit
Immobilized enzyme and affinity resin for above hinge digestion of human IgG1 and purification of Fab fragments
FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact and homogenous Fab and Fc fragments.
FabALACTICA Fab Kit consists of spin columns of FabALACTICA Immobilized for antibody digestion, and spin columns of CaptureSelect™* Fc resin for purification of the Fab fragments. One spin column contains sufficient FabALACTICA-coupled agarose beads to process 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
Why FabALACTICA™ Fab Kit?
- Purification of homogeneous Fab fragments from human IgG1
- Reliable workflow for preparative applications
- Enables chain pairing characterization of bispecific antibodies
Available Products
FabALACTICA™ Fab Kit Microspin 0.5 mg
Immobilized enzyme and affinity resin for above hinge digestion of 0.5 mg human IgG1 and purification of fragments
FabALACTICA™ Fab kit Microspin 5 x 0.5 mg
Immobilized enzyme and affinity resin for above hinge digestion of 5 x 0.5 mg human IgG1 and purification of fragments
FabALACTICA™ Fab Kit Midispin 10 mg
Immobilized enzyme and affinity resin for above hinge digestion of 10 mg human IgG1 and purification of fragments
FabALACTICA™ Fab Kit Maxispin 100 mg
Immobilized enzyme and affinity resin for above hinge digestion of 100 mg human IgG1 and purification of fragments
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
Content and Storage
The FabALACTICA Immobilized spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Performance Activity
Simple preparation of highly pure Fab fragments from IgG1 antibodies using FabALACTICA digestion combined with Fc affinity purification.
Citations
Powered by BiozFAQ and Support
Can I digest Fc-fusion proteins using FabALACTICA (IgdE)?
No, FabALACTICA has shown very poor activity on Fc-fusion proteins.
We want to make Fab’s directly from antibodies. When would the selection of FabALACTICA be the first choice over GingisKHAN or FabULOUS?
FabALACTICA does not require reducing conditions as both GingisKHAN and FabULOUS does, and allows for generation of Fab fragments from native human IgG1.
Can I release Fc fragments from the CaptureSelect column after isolating the Fab fragments?
It is not recommended to elute the Fc fragments from the CaptureSelect column since the fraction might also include undigested or semi digested material.
Can I re-use FabALACTICA Immobilized columns?
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
What is the difference between FabALACTICA and GingisKHAN?
FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (…KSCDKT / HTCPPCP…) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (…KSCDK/THTCPPCP…).
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
* Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
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