FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at one single amino acid position above the hinge generating intact Fc and Fab fragments. FabALACTICA is available as lyophilized powder for digestion of 2 mg of human IgG1 without the need of reducing conditions.
| Application | |
|---|---|
| Product Group | |
| Source/Origin | Cloned from S. agalactiae, Produced in E. coli (His-tagged), 70 kDa |
| Product Composition | |
| Digestion Site | |
| Specificity | |
| Substrates | |
| Reaction Conditions | 16-18 hour reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
| Buffer Compatibility |
Yes, for human IgG1 molecules both enzymes can be used in combination to generate a specific hinge peptide. The incubation time is significantly longer for FabALACTICA and it is recommended to first run the FabALACTICA digest and then add FabRICATOR for the last 30 min.
No, FabALACTICA has shown very poor activity on Fc-fusion proteins.
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
It is not recommended to elute the Fc fragments from the CaptureSelect column since the fraction might also include undigested or semi digested material.
We recommend using FabALACTICA Immobilized for digesting human IgG1 and generating a final preparation free of any residual enzyme. You can also use FabALACTICA Fab kit for digestion of human IgG1 and purification of the generated Fab fragments.
FabALACTICA does not require reducing conditions as both GingisKHAN and FabULOUS does, and allows for generation of Fab fragments from native human IgG1.
FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (…KSCDKT / HTCPPCP…) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (…KSCDK/THTCPPCP…).
