OglyZOR endoglycosidase (O-Glycosidase) specifically hydrolyzes O-glycans of core 1 and core 3 disaccharides on native glycoproteins. The novel OglyZOR enzyme technology is highly efficient and easy to use compared to other similar enzymes on the market.

OglyZOR is supplied as a lyophilized powder and is available in 2000 unit vials for deglycosylation of 2 mg glycoprotein. It requires removal of sialic aids for activity, and is therefore provided with 2000 units SialEXO® (sialidase).

Detailed Information

OglyZOR is an endoglycosidase (endo-α-N-acetylgalactosaminidase) that catalyzes the hydrolysis of core 1 and core 3 O-glycans (disaccharides) from native glycoproteins. The sialic acids of the glycans should be removed for OglyZOR enzyme activity. The SialEXO, composed of a mix of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds, is used together with OglyZOR for efficient removal of the O-linked disaccharides. Optimal activity is obtained at pH 6.5 to 7.5 and at 37 °C.

OglyZOR is derived from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in E. coli. The SialEXO-enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.

Unit Definition

One unit of OglyZOR digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFαR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

The OglyZOR box includes:

  • 1 vial of OglyZOR (G1-OG1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

  • 1 vial of SialEXO (G1-SM1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

The OglyZOR box is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution, the enzymes are stable for 1 month at +4-8 °C.

Key Characteristics

  • Hydrolyzes O-glycans on glycoproteins
  • 2-4h reaction
  • Requires removal of sialic acids using SialEXO® (included)
  • Core 1 and core 3 type O-glycan disaccharides


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Antibody Characterization

Download Poster

"Novel Enzymes for O-glycan Analysis", CASSS 2018

How to Order

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Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.





Product Specification



Safety Data Sheet



Certificate of Analysis

FAQ and Support

Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.
No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).
OglyZOR works only on desialylated O-glycans and a sialidase (SialEXO) is included with the product. This sialidase is not O-glycan specific but will also desialylate any N-glycans present. 
Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions over night at pH ~7.0. 
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Patent and Disclaimer


All rights reserved. Aspects of OglyZOR® technology are encompassed by pending patent applications in the name of Genovis AB.

The trademark OglyZOR® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.