SmartEnzymes™

OglyZOR workflow

OglyZOR®

OglyZOR endoglycosidase (O-Glycosidase) specifically hydrolyzes O-glycans of core 1 and to some extent core 3 disaccharides on native glycoproteins. The novel OglyZOR enzyme technology is highly efficient and easy to use compared to other similar enzymes on the market.

The enzyme is supplied as a lyophilized powder and is available in 2000 unit vials for deglycosylation of 2 mg glycoprotein. It requires removal of sialic aids for activity, and is therefore provided with 2000 units SialEXO® (sialidase).

Detailed Information

OglyZOR is an endoglycosidase (endo-α-N-acetylgalactosaminidase) that catalyzes the hydrolysis of core 1 and to some extent core 3 O-glycans (disaccharides) from native glycoproteins. The sialic acids of the glycans should be removed for OglyZOR enzyme activity. The SialEXO, composed of a mix of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds, is used together with OglyZOR for efficient removal of the O-linked disaccharides. Optimal activity is obtained at pH 6.5 to 7.5 and at 37 °C.

OglyZOR is derived from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in E. coli. The SialEXO-enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.

Unit Definition

One unit digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFαR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

The box includes:

  • 1 vial of OglyZOR (G1-OG1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.
  • 1 vial of SialEXO (G1-SM1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

The box is shipped at ambient temperature, and should be stored at -20°C upon arrival.
After reconstitution, the enzymes are stable for 1 month at +4-8 °C.

SmartStories

Analytical services lab exploit OglyZOR, FragIT and SialEXO

Key Characteristics

  • Hydrolyzes O-glycans on glycoproteins
  • 2-4h reaction
  • Requires removal of sialic acids using SialEXO® (included)
  • Core 1 type O-glycan disaccharides

Applications

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Antibody Characterization

Download Poster

"Novel Enzymes for O-glycan Analysis", CASSS 2018

How to Order

For details on ordering please visit How to order. Click add to cart above to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

Documents

Instructions

Product Specification

Safety Data Sheet

 

 

Certificate of Analysis

FAQ and Support

Popular FAQ

OglyZOR works only on desialylated O-glycans and a sialidase (SialEXO) is included with the product. This sialidase is not O-glycan specific but will also desialylate any N-glycans present.

No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).

Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.

Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions over night at pH ~7.0.

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Legal and Disclaimers

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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