OglyZOR

OglyZOR

OglyZOR™

OglyZOR is aendoglycosidase that specifically hydrolyzes O-glycans of core 1 and core 3 disaccharides on native glycoproteins.

OglyZOR is supplied as a lyophilized powder and is available in 2000 unit vials for deglycosylation of 2 mg glycoprotein. It requires removal of sialic aids for activity, and is therefore provided with 2000 units SialEXO™ (sialidase).

 

 

Detailed Information

OglyZOR is an endoglycosidase (endo-α-N-acetylgalactosaminidase) that catalyzes the hydrolysis of core 1 and core 3 O-glycans (disaccharides) from native glycoproteins. The sialic acids of the glycans should be removed for OglyZOR enzyme activity. The SialEXO, composed of a mix of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds, is used together with OglyZOR for efficient removal of the O-linked disaccharides. Optimal activity is obtained at pH 6.5 to 7.5 and at 37 °C.

OglyZOR is derived from Streptococcus oralis and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 227 kDa. SialEXO is derived from Akkermansia muciniphila and expressed in E. coli. The SialEXO-enzymes contain His-tags and the molecular weights of the components are 42 kDa and 66 kDa, respectively.

Unit Definition

One unit of OglyZOR digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFαR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

The OglyZOR box includes:

  • 1 vial of OglyZOR (G1-OG1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

  • 1 vial of SialEXO (G1-SM1-020) supplied lyophilized in TBS pH 7.6, with no preservatives added.

The OglyZOR box is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution, the enzymes are stable for 1 month at +4-8 °C.

 Download Brochure

O-glycans_Produktfolder_Webb_20170601_OG1706-1

 

Applications

  • Removal of O-glycans for glycan analysis
  • Confirmation of O-glycan presence
  • Reduce sample heterogeneity

 

Key Characteristics

  • Endoglycosidase acting on O-glycans
  • Active on native glycoproteins
  • Specific for core 1 and core 3 O-glycan disaccharides
  • Requires pretreatment with SialEXO

How to order

For details on ordering please visit How to order. Click add to cart above to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

Documents

Instructions

 

 

Product specifications

 

 

Safety Data Sheet

 

 

FAQ and Support

Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.

Find all FAQ here

Patent and Disclaimer

OglyZOR™

All rights reserved. Aspects of OglyZOR™ technology are encompassed by pending patent applications in the name of Genovis AB.

The trademark OglyZOR™ is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2017 Genovis AB.