SialEXO

SialEXO

SialEXO™

SialEXO is a sialidase mix for complete removal of sialic acids from native glycoproteins.

SialEXO is supplied as a lyophilized powder with no preservatives added. It is available in 2000 unit vials for release of sialic acids on 2 mg glycoprotein.

 

 

Detailed Information

SialEXO is a combination of two sialidases for efficient desialylation of O- and N-glycosylated proteins. The mix is composed of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds.

SialEXO hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 6.5 to 9.

Enzymes in SialEXO are derived from Akkermansia muciniphila and expressed in E. coli. The enzymes in the mix contain a His-tag and the molecular weights of the components are 43 kDa and 66 kDa, respectively.

Unit Definition

One unit of SialEXO hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

SialEXO is supplied lyophilized in TBS  pH 7.6, with no preservatives added.
SialEXO is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution SialEXO is stable for 1 month at +4-8 °C.

Download Brochure

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Applications

  • Removal of all sialic acids
  • Pretreatment prior to OpeRATOR incubation
  • Pretreatment prior to OglyZOR deglycosylation
  • Exoglycosidase array

 

Key Characteristics

  • Acts on native glycoproteins
  • Cleaves α2-3, α2-6 or α2-8 linked sialic acids
  • Hydrolyzes sialic acids within 2 h
  • Acts on both N- and O-linked glycans
  • Compatible with OpeRATOR and OglyZOR

How to order

For details on ordering please visit How to order. Click add to cart above to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

Documents

Instructions

 

 

Product specifications

 

 

Safety Data Sheet

 

 

Patent and Disclaimer

SialEXO™

All rights reserved. Aspects of SialEXO™ technology are encompassed by pending patent applications in the name of Genovis AB.

The trademark SialEXO™ is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2017 Genovis AB.