Powered by BiozGlycOCATCH™
Download Product Folder
GlycOCATCH™ – Enrichment of O-glycopeptides
GlycOCATCH is an affinity resin for purification of mucin-type O-glycosylated proteins and peptides. The affinity resin is based on inactive OpeRATOR® enzyme that has been engineered to bind O-glycosylated proteins and peptides with high affinity.
SmartEnzymes™
Applications of GlycOCATCH include specific enrichment or removal of O glycoproteins and peptides, glycomics, studies of complex samples and characterization of biopharmaceuticals.
Why GlycOCATCH™?
- Specific enrichment of mucin-type O-glycosylated proteins and peptides
- Reliable – high affinity binding
- Fast and easy-to-use workflow
Mucin-type O-glycosylated proteins and peptides
2 h workflow
Desialylation using SialEXO® (included) increases the binding
Binds to glycoproteins and peptides carrying mucin-type O-glycans
Product Formats
GlycOCATCH™ Affinity Purification
Enrichment resin for affinity purification of mucin-type O-glycoproteins and peptides
Buy product
About GlycOCATCH™
GlycOCATCH is designed to specifically bind mucin-type O-glycosylated proteins and peptides. The affinity resin is based on the inactive OpeRATOR enzyme, that has been engineered to bind O-glycosylated proteins and peptides with high affinity.
The binding is performed in physiological buffers at pH 5-8. Due to the strong interaction between the GlycOCATCH resin and O-glycoproteins/peptides, the elution can be performed with 8 M urea. Alternatively, the elution can be performed with the active OpeRATOR enzyme, that digests the bound O-glycoproteins N-terminally of the O-glycan sites. In the latter case, peptides are recovered under native conditions. The OpeRATOR Lyophilized enzyme is included in the purchase. Since asialylated O-glycans on O-glycoproteins are bound more efficiently to the resin, the sialidase mix SialEXO Lyophilized is also included in the purchase.
Applications
Selective enrichment of O-glycoproteins from human serum for improved LC-MS/MS detection and glycosylation-focused proteomic analysis.
Selective enrichment of IgA O-glycopeptides for improved detection and characterization of hinge-region glycosylation.
Citations
Resources
SmartEnzymes™ for O-glycosylation Analysis using Mass Spectrometry
Download Scientific Poster
FAQ and Support
When and why should I use the OpeRATOR elution procedure?
The OpeRATOR enzyme digests O-glycosylated proteins N-terminally to the serine (S) and threonine (T) glycosylation sites. OpeRATOR therefore facilitates mapping of O-glycosylation sites by LC-MS and as the digested products no longer bind to the GlycOCATCH resin, elution and sample preparation can be achieved in a single step. However, this requires the O-glycosylation sites to be spaced closely together for the OpeRATOR digestion to yield peptides of a size suitable for analysis by LC-MS/MS.
Is it possible to digest the glycoprotein with OpeRATOR first and then enrich the O-glycopeptides on GlycOCATCH?
No, when the glycoprotein is digested with OpeRATOR, the binding capacity of GlycOCATCH is lost.
Can I load a digested protein solution directly onto the GlycOCATCH column?
Yes. IMPORTANT! Before you load your sample make sure that the protease in solution is inactivated and that the pH, buffer, chaotrope and NaCl concentrations are within the recommended ranges according to the instructions.
Does GlycOCATCH bind all O-glycosylated proteins and peptides?
No, GlycOCATCH is designed to bind specifically to mucin type (core 1) O-glycosylated proteins and peptides. The performance is significantly enhanced by removal of sialic acids using SialEXO.
Legal & Disclaimers
All rights reserved. Genovis products may be covered by one or more patents, trademarks and copyrights owned by Genovis AB or licensed from third parties. For more information about commercial rights, please contact the Genovis team at licensing@genovis.com.
Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
© Genovis AB