GingisKHAN® (Kgp)
GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity and the reaction is performed in the presence of 2 mM cystein at neutral pH.
GingisKHAN is available in two product formats:
- GingisKHAN Enzyme - lyophilized enzyme and reducing agent
- GingisKHAN Fab kit - lyophilized enzyme and reducing agent together with affinity purification resin for easy generation and purification of Fab fragments from human IgG1.
Product Information
GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. The reducing agent (2 mM cysteine) is provided together with the enzyme.
GingisKHAN is purified from Porphyromonas gingivalis.
Key Characteristics
- Human IgG1
- 60 min reaction
- 2 mM cysteine (included)
- KSCDK / THTCPPCP (above the hinge)
Applications
- Generation of Fab fragments from human IgG1
- Subunit LC-MS analysis
- Characterize heavy and light chain pairing of bispecific antibodies
- Study monovalent binding of Fab fragments to the antigen
- Epitope mapping using HDX-MS
- Cystallization of Fab fragments
Product Formats
GingisKHAN® Enzyme
GingisKHAN enzyme supplied as lyophilized powder together with 5 vials of lyophilized GingisKHAN reducing agent for convenient and reliable performance. The enzyme is available in 2000 unit vials for digestion of up to 2 mg human IgG1.
GingisKHAN® Fab kit
GingisKHAN Fab kit is used for rapid and easy preparation and purification of of Fab fragments from human IgG1 antibodies. The kit consists of two parts; lyophilized GingisKHAN enzyme for digestion of human IgG1 and affinity spin columns for purification of intact Fab fragments.
Latest References using this Product
- Accurate Sequence Analysis of a Monoclonal Antibody by Top- Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
2018-06-12
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D. Compton, Neil L. Kelleher - Cysteine-SILAC Mass Spectrometry Enabling the Identification and Quantitation of Scrambled Interchain Disulfide Bonds: Preservation of Native Heavy-Light Chain Pairing in Bispecific IgGs Generated by Controlled Fab-arm Exchange
2017-09-18
Ewald T. J. van den Bremer, Aran F. Labrijn, Ramon van den Boogaard, Patrick Priem, Kai Scheffler, Joost P. M. Melis, Janine Schuurman, Paul W. H. I. Parren and Rob N. de Jong
Brochures and Posters
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Product Documents
Patent and Disclaimer
GingisKHAN®
The trademark GingisKHAN is the property of Genovis AB.
For research use only. Not intended for any animal or human therapeutic diagnostic use.
©2018 Genovis AB.