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GingisKHAN® (Kgp)

GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity and the reaction is performed in the presence of 2 mM cystein at neutral pH.

 

GingisKHAN is available in two product formats:

  • GingisKHAN Enzyme - lyophilized enzyme and reducing agent
  • GingisKHAN Fab kit - lyophilized enzyme and reducing agent together with affinity purification resin for easy generation and purification of Fab fragments from human IgG1. 

Product Information

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. The reducing agent (2 mM cysteine) is provided together with the enzyme.

GingisKHAN is purified from Porphyromonas gingivalis.

Key Characteristics

  • Human IgG1
  • 60 min reaction
  • 2 mM cysteine (included)
  • KSCDK / THTCPPCP (above the hinge)

Applications

  • Generation of Fab fragments from human IgG1
  • Subunit LC-MS analysis
  • Characterize heavy and light chain pairing of bispecific antibodies
  • Study monovalent binding of Fab fragments to the antigen
  • Epitope mapping using HDX-MS
  • Cystallization of Fab fragments

Product Formats

GingisKHAN® Enzyme

GingisKHAN enzyme supplied as lyophilized powder together with 5 vials of lyophilized GingisKHAN reducing agent for convenient and reliable performance. The enzyme is available in 2000 unit vials for digestion of up to 2 mg human IgG1.

GingisKHAN® Fab kit

GingisKHAN Fab kit is used for rapid and easy preparation and purification of of Fab fragments from human IgG1 antibodies. The kit consists of two parts; lyophilized GingisKHAN enzyme for digestion of human IgG1 and affinity spin columns for purification of intact Fab fragments.

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Applications

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Antibody Characterization

CASSS 2015

"Initial Characterization of a Novel Cysteine Protease for Antibody Subunit Analysis"

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Patent and Disclaimer

GingisKHAN®

The trademark GingisKHAN is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic diagnostic use.
©2018 Genovis AB.