



GingisKHAN® (Kgp)
GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity and the reaction is performed in the presence of 2 mM cysteine at neutral pH.
GingisKHAN is available in two product formats:
- GingisKHAN Enzyme - lyophilized enzyme and reducing agent
- GingisKHAN Fab kit - lyophilized enzyme and reducing agent together with affinity purification resin for easy generation and purification of Fab fragments from human IgG1.


Product Information
GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. The reducing agent (2 mM cysteine) is provided together with the enzyme.
GingisKHAN is purified from Porphyromonas gingivalis.
Key Characteristics
- Human IgG1
- 60 min reaction
- 2 mM cysteine (included)
- KSCDK / THTCPPCP (above the hinge)
Applications
- Subunit LC-MS analysis
- Characterize heavy and light chain pairing of bispecific antibodies
- Study monovalent binding of Fab fragments to the antigen
- Epitope mapping using HDX-MS
- Cystallization of Fab fragments
Product Formats
GingisKHAN® Enzyme
GingisKHAN enzyme supplied as lyophilized powder together with 5 vials of lyophilized GingisKHAN reducing agent for convenient and reliable performance. The enzyme is available in 2000 unit vials for digestion of up to 2 mg human IgG1.
GingisKHAN® Fab kit
GingisKHAN Fab kit is used for rapid and easy preparation and purification of of Fab fragments from human IgG1 antibodies. The kit consists of two parts; lyophilized GingisKHAN enzyme for digestion of human IgG1 and affinity spin columns for purification of intact Fab fragments.
FabALACTICA Brochures, Posters and Applications
Brochures and Posters
Download Genovis brochures and posters with the latest details on the SmartEnzymes
Download GingisKHAN Poster and External References
Poster from AT Europe 2014
"Initial Characterization of a Novel Cysteine Protease for Antibody Subunit Analysis"
FAQ
Is GingisKHAN an IgG specific protease?
How can I stop the GingisKHAN reaction?
I already used the 5 reducing agents supplied with the enzyme, can I use another reducing agent besides cysteine?
Can you recommend a protocol for purification of the Fab?
Find all FAQ here
Product Documents
Legal and Disclaimers
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For more information about commercial rights, please contact Genovis team at info@genovis.com.
Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.