GingisKHAN® (Kgp)
GingisKHAN (Kgp) is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity and the reaction is performed in the presence of 2 mM cystein at neutral pH.
GingisKHAN is available in two product formats:
- GingisKHAN Enzyme - lyophilized enzyme and reducing agent
- GingisKHAN Fab kit - lyophilized enzyme and reducing agent together with affinity purification resin for easy generation and purification of Fab fragments from human IgG1.
Product Information
GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. The reducing agent (2 mM cysteine) is provided together with the enzyme.
GingisKHAN is purified from Porphyromonas gingivalis.
Key Characteristics
- Human IgG1
- 60 min reaction
- 2 mM cysteine (included)
- KSCDK / THTCPPCP (above the hinge)
Applications
- Generation of Fab fragments from human IgG1
- Subunit LC-MS analysis
- Characterize heavy and light chain pairing of bispecific antibodies
- Study monovalent binding of Fab fragments to the antigen
- Epitope mapping using HDX-MS
- Cystallization of Fab fragments
Product Formats
GingisKHAN® Enzyme
GingisKHAN enzyme supplied as lyophilized powder together with 5 vials of lyophilized GingisKHAN reducing agent for convenient and reliable performance. The enzyme is available in 2000 unit vials for digestion of up to 2 mg human IgG1.
GingisKHAN® Fab kit
GingisKHAN Fab kit is used for rapid and easy preparation and purification of of Fab fragments from human IgG1 antibodies. The kit consists of two parts; lyophilized GingisKHAN enzyme for digestion of human IgG1 and affinity spin columns for purification of intact Fab fragments.
Latest References using this Product
- Multiplexed Middle-Down Mass Spectrometry Reveals Light and Heavy Chain Connectivity in a Monoclonal Antibody
2018-09-25
Kristina Srzentic, Konstantin O. Nagornov, Luca Fornelli, Anna A. Lobas, Daniel Ayoub, Anton Kozhinov, Natalia Gasilova, Laure Menin, Alain Beck, Mikhail Gorshkov, Konstantin Aizikov, and Yury O. Tsybin - Accurate Sequence Analysis of a Monoclonal Antibody by Top- Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
2018-06-12
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D. Compton, Neil L. Kelleher
Brochures and Posters
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Product Documents
Patent and Disclaimer
GingisKHAN®
The trademark GingisKHAN is the property of Genovis AB.
For research use only. Not intended for any animal or human therapeutic diagnostic use.
©2018 Genovis AB.