FAQ
Is GingisKHAN an IgG specific protease?
No, GingisKHAN is a general protease. However, under the recommended reaction conditions, it digests native human IgG1 at one specific site above the hinge.
How can I stop the GingisKHAN reaction?
The enzyme is a cysteine protease that can be inhibited by alkylating agents such as iodoacetamide. There are also several other known inhibitors to this enzyme, like KYT-36.
I already used the 5 reducing agents supplied with the enzyme, can I use another reducing agent besides cysteine?
The ready-to-use reaction buffer additive has been optimized to provide full enzymatic activity without affecting the disulphide bridges between the light chain and Fd region of the heavy chain, keeping the Fab intact. We recommend preparing a fresh solution of pH neutral cysteine, for use the same day, with a final reaction concentration of 2mM.
Can you recommend a protocol for purification of the Fab?
Genovis provides a GingisKHAN Fab kit containing a specific Capture Select CH1 resin for further purification of the Fab. See
this page for more information.
