FabDELLO is a protease that digests human IgG1 at a single site above the hinge, yielding intact Fab and Fc fragments within two hours, with no need for reducing conditions.
The enzyme is active on antibodies with mutated hinge regions, such as the LALA mutation, and enables middle-level LC-MS for characterization of critical quality attributes on antibodies with mutated hinges.
Robust digestion of hinge-mutated antibodies, including LALA-mutated antibodies
Fast - generates intact Fab and Fc fragments from human IgG1 within two hours
Lyophilized enzyme for above hinge digestion of human IgG1
About FabDELLO™
FabDELLO digests human IgG1 at a single site above the hinge (...KSCDK / THTCPPCP…), generating intact Fab and Fc fragments.
FabDELLO is a lysine-specific protease. The single digestion site is a result of the three-dimensional structure of human IgG1 making this lysine exposed for the enzyme. Additional digestion sites at exposed lysines on the Fc may appear if the N-glycans are removed. FabDELLO is active under native conditions and requires the presence of calcium ions. Optimal activity is obtained at 37°C and pH 7-8.5.
FabDELLO is cloned from Bdellovibrio bacteriovorus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 32 kDa.
FabDELLO™ Lyophilized
Lyophilized enzyme for above hinge digestion of human IgG1.
It is available as a lyophilized powder in 8 x 100 units or 96 x 100 units strip wells, each well digesting 100 µg human IgG1. FabDELLO is supplied with 1 vial of 20x CaCl2 solution (200 mM).
Flexible format for method development
Possible to combine enzymes for improved efficiency
Amenable to automated workflows
Ready to use – just add water!
Available Products
FabDELLO Lyophilized 8 x 100 unitsLyophilized enzyme for above hinge digestion of 8 x 100 𝜇g human IgG1B1-BD1-008€421.00Buy / Request a Quote
FabDELLO Lyophilized 96 x 100 unitsLyophilized enzyme for above hinge digestion of 96 x 100 𝜇g human IgG1B1-BD1-096€2,346.00Buy / Request a Quote
One unit FabDELLO digests ≥ 90% of 1 µg human IgG1, when incubated in Tris buffered saline (TBS), pH 7.6, containing 10 mM CaCl2, at 37°C for 2 h.
Content and Storage
FabDELLO Lyophilized is supplied in 50 mM Tris-HCl, 150 mM NaCl, pH 7.6, with no preservatives added. It is available in 8 x 100 units or 96 x 100 units strip wells, each well digesting 100 µg human IgG1. FabDELLO is supplied with 1 vial 20x CaCl2 solution (200 mM).
FabDELLO is shipped cold and should be stored at -20°C upon arrival. Since FabDELLO has autoproteolytic activity, the enzyme should be kept cold and used the same day as reconstitution.
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