A simple and rapid estimation of IgG core fucosylation using LC-MS analysis.
Deglycosylation
Deglycosylation involves the removal of glycans to simplify analysis and reveal structural details of complex proteins. It is commonly used to confirm glycosylation sites and improve mass spectrometry data. Our solutions support efficient and reproducible deglycosylation for clear and reliable results.
Applications
A simple and rapid estimation of IgG core fucosylation and glycan occupancy using LC-MS analysis.
O-glycan site occupancy profiling and complete deglycosylation of etanercept for in-depth characterization of complex O-glycoproteins.
Confirmation and quantification of Tn antigens on O-glycosylated biopharmaceuticals while simplifying intact-mass analysis and revealing protein variants.
Removal of glycan β-galactose to simplify IgG glycan heterogeneity, improve LC-MS analysis, and reveal underlying glycation modifications.
Complete Fc N-glycan removal under native conditions, enabling simplfied subunit LC-MS analysis of therapeutic antibodies.
Comprehensive N-glycan profiling using released glycan workflow, supporting sensitive detection and monitoring of critical quality attributes.
Removal of N- and O-glycans from complex and heavily glycosylated proteins, enables clear, interpretable LC-MS analysis.
Native removal of O-glycans and removal of inaccessible N-glycans under denaturing conditions, enables complete glycan analysis of the complex C1-inhibitor
Resources
SmartEnzymes™ Brochure
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