SEQguard™ Dino Preserve
Transport medium for purified RNA, enabling ambient temperature shipment to Plasmidsaurus for RNA-seq analysis.
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SEQguard™ – Dino Preserve
SEQguard Dino Preserve is a transport medium optimized in partnership with Plasmidsaurus. It enables ambient temperature shipment of purified RNA samples for sequencing via the Plasmidsaurus pipeline.
Genomics
It tolerates a wide temperature range, making it ideal for shipment protection year-round. SEQguard Dino Preserve eliminates the need for dry ice, saving costs and hassle while providing a more sustainable alternative. One 150 μl vial is sufficient to stabilize 24 purified RNA samples when used according to Plasmidsaurus’ usage guidelines.
Why SEQguard™?
- No more dry ice! Ambient transport with room temperature stability
- Cost-efficient – reduce shipping costs by eliminating dry ice
- Faster turnaround – simplified logistics for quicker results
- Preserves sample integrity – protects RNA from purification to sequencing
- Optimized for RNA-seq – tailored for best performance in the Plasmidsaurus pipeline
Maintains stability under light exposure
Transport medium for extracted RNA, enabling shipment of purified RNA to Plasmidsaurus for RNA sequencing
Stored at room temperature (15-30°C)
Product Formats
How it Works
Transport medium for purified RNA, enabling ambient temperature shipment to Plasmidsaurus for RNA-seq analysis.
SEQguard Dino Preserve is supplied as a solution in vials containing 150 μl liquid. Each 150 μl vial is sufficient to stabilize 24 purified RNA samples when used according to Plasmidsaurus’ usage guidelines.
Partners
Plasmidsaurus offers ultrafast RNA-Seq service, transforming gene expression analysis with unprecedented speed, affordability, and convenience. Check out and order RNA-Seq from Plasmidsaurus here.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »FAQ and Support
Can you tell me how long RNA is stable in this buffer in ambient temperature, +4°C, – 20°C, and – 80°C?
Ambient temperature: RNA in SEQguard has been shown to maintain high quality for Plasmidsaurus library preparation for 7 days, often longer. However, shipping times should still be minimized. Typical transit times are routinely acceptable without issue.
+4°C: Provides longer stability than ambient temperature.
−20°C: Suitable for storage over weeks to months.
−80°C: Suitable for long-term storage (effectively indefinite).
Once stored in this buffer, can I use the RNA for cDNA prep followed by qPCR or one step qPCR or one step RT-ddPCR directly?
At present, this guidance is based on Plasmidsaurus RNA-seq processing, as this is the context in which the product has been evaluated. If alternative downstream methods are used, performance should be validated independently.
RNA stability is also influenced by the quality of sample extraction and purification. High-quality RNA preparations (i.e., low RNase contamination and minimal metal ion presence) are associated with improved stability over time in SEQguard, whereas lower-quality preparations may result in faster degradation.
RNA should be eluted in a low-EDTA TE buffer (10 mM Tris, 0.1 mM EDTA), such as IDTE buffer (IDT, pH 7.5).
On Plasmidsaurus website, it’s indicated that: Mix 24 μL of RNA in IDTE buffer with 6 μL of SEQguard Dino Preserve to achieve a final concentration between 10-100 ng/μL. If the RNA is eluted in nuclease-free water – can I still mix it with SEQguard at this ratio?
Samples can be submitted to Plasmidsaurus in either nuclease-free water supplemented with SEQguard Dino Preserve (mixed at the same ratio recommended for IDTE buffer: 24 μL RNA with 6 μL SEQguard Dino Preserve) or in IDTE buffer (10 mM Tris, 0.1 mM EDTA, pH 7.5).
Submission in IDTE buffer is recommended, as the addition of EDTA to a final concentration of up to 0.1 mM has been shown to improve RNA stability at elevated temperatures. Samples submitted in nuclease-free water will still be processed.
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