Frequently Asked Questions

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Once stored in this buffer, can I use the RNA for cDNA prep followed by qPCR or one step qPCR or one step RT-ddPCR directly?

At present, this guidance is based on Plasmidsaurus RNA-seq processing, as this is the context in which the product has been evaluated. If alternative downstream methods are used, performance should be validated independently.

RNA stability is also influenced by the quality of sample extraction and purification. High-quality RNA preparations (i.e., low RNase contamination and minimal metal ion presence) are associated with improved stability over time in SEQguard, whereas lower-quality preparations may result in faster degradation.

RNA should be eluted in a low-EDTA TE buffer (10 mM Tris, 0.1 mM EDTA), such as IDTE buffer (IDT, pH 7.5).

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On Plasmidsaurus website, it’s indicated that: Mix 24 μL of RNA in IDTE buffer with 6 μL of SEQguard Dino Preserve to achieve a final concentration between 10-100 ng/μL. If the RNA is eluted in nuclease-free water – can I still mix it with SEQguard at this ratio?

Samples can be submitted to Plasmidsaurus in either nuclease-free water supplemented with SEQguard Dino Preserve (mixed at the same ratio recommended for IDTE buffer: 24 μL RNA with 6 μL SEQguard Dino Preserve) or in IDTE buffer (10 mM Tris, 0.1 mM EDTA, pH 7.5).

Submission in IDTE buffer is recommended, as the addition of EDTA to a final concentration of up to 0.1 mM has been shown to improve RNA stability at elevated temperatures. Samples submitted in nuclease-free water will still be processed.

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Can you tell me how long RNA is stable in this buffer in ambient temperature, +4°C, – 20°C, and – 80°C?

Ambient temperature: RNA in SEQguard has been shown to maintain high quality for Plasmidsaurus library preparation for 7 days, often longer. However, shipping times should still be minimized. Typical transit times are routinely acceptable without issue.

+4°C: Provides longer stability than ambient temperature.

−20°C: Suitable for storage over weeks to months.

−80°C: Suitable for long-term storage (effectively indefinite).

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