ac-cleavage-galexo-04-outline hydrolysis of galactose


GalactEXO is a β-galactosidase for efficient hydrolysis of galactose residues from N- and O-linked glycans. The GalactEXO galactosidase display broad activity on β1-3 and β1-4 galactose on N- or O-glycosylated proteins or free oligosaccharides.

GalactEXO is available as a 2000 units lyophilized galactosidase mix for hydrolysis of galactose from 2 mg glycoprotein in 2h.

Key Characteristics

  • Galactosidase hydrolyzing galactose on N- and O-glycoproteins or free oligosaccharides
  • β1-3 and β1-4 linked galactose residues
  • 2 h reaction
  • Requires no co-factors

Detailed Information

GalactEXO is a mix of β-galactosidases for efficient removal of galactose residues on N- and O-glycosylated proteins or oligosaccharides. The mix is composed of two galactosidases for highly efficient hydrolysis of both β1-3 and β1-4 linked galactoses. GalactEXO hydrolyzes glycoproteins under native conditions and is highly activity in a broad pH range, 5.5 to 7.5. The enzymes in GalactEXO are derived from Akkermansia muciniphila and expressed in E. coli. GalactEXO is composed of two galactosidases both with His-tags and molecular weights of the components are 87 kDa and 109 kDa, respectively.


Unit Definition

One unit of GalactEXO hydrolyzes galactoses from >95% of 0.15 nmol Gal-β1,3-GalNAc-pNP and 2 nmol Gal-β1,4-GlcNAc-pNP when incubated in 20 mM Tris pH 6.8 at 37°C for 30 min.

1 unit of GalactEXO will hydrolyze the galactose of 1 ug of glycoprotein.


Content and Storage

GalactEXO is supplied lyophilized in TBS pH 7.6, with no preservatives added.
GalactEXO is shipped cold and should be stored at -20 °C upon arrival.
After reconstitution GalactEXO is stable for 1 month at +4-8 °C.



Product Specification

Safety Data Sheet

Certificate of Analysis



Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.
Yes, both beta1-3 linked galactose on O-glycans and beta1-4 galactose on N-glycans are hydrolyzed by GalactEXO.
For most substrates such as antibodies or Fc-fusion proteins a 2 h incubation results in hydrolysis of over 95% of the present galactose. More complex substrates with high degree of galactosylation may require longer incubation times.

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GalactEXO Brochure

Removal of galactose residues using GalactEXO – a broad acting β-galactosidase


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