Site-specific Free Thiol Analysis by HILIC-MS with SmartEnzymes™

GenovisWebinars - Development of a Novel Subunit HILIC-MS Method for Site-specific Localization and Quantitation of Intra-domain Free Thiols

We are excited to welcome Xin Wang from Regeneron Pharmaceuticals to share how the reliability and robust performance of Genovis’ SmartEnzymes have enabled the subunit HILIC-MS method to be successfully applied across multiple Regeneron programs and commercial mAbs. This broad applicability has validated its exceptional utility as a label-free approach for quantitating free thiols in mAb analysis.

Key Learning Objectives

Learn how FabRICATOR and GalactEXO enable precise identification and localization of intra-chain free thiols in mAbs
Learn how middle-up HILIC-MS can be used as a label-free method for free thiol quantitation.
Explore the successful application of this method across various Regeneron programs and seven commercial mAbs.