GalactEXO®
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GalactEXO® Immobilized
Immobilized enzyme for hydrolysis of β1-3,4-linked galactose in spin columns
GalactEXO is a β-Galactosidase mix that hydrolyses β1-3,4-linked terminal galactose on N- and O-glycosylated proteins or oligosaccharides.
The GalactEXO Immobilized spin columns contain GalactEXO enzyme covalently coupled to agarose beads, for hydrolysis of β1-3,4-linked galactose on glycoproteins without contaminating the final preparation with enzyme.
The Microspin columns are available for hydrolysis of 5 or 10 x 0.5 mg glycoprotein.
Why GalactEXO® Immobilized?
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
GalactEXO® Immobilized Microspin 5 x 0.5 mg
Immobilized enzyme for hydrolysis of β1-3,4-linked galactose on 5 x 0.5 mg glycoprotein in spin columns
GalactEXO® Immobilized Microspin 10 x 0.5 mg
Immobilized enzyme for hydrolysis of β1-3,4-linked galactose on 10 x 0.5 mg glycoprotein in spin columns
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
One column of GalactEXO Immobilized catalyzes the hydrolysis of galactoses from ≥ 95% of 41 μmol 4-nitrophenyl-β-D-galactopyranoside when incubated in 20 mM Tris, 10% ethanol pH 6.8 at room temperature for 15 minutes, as monitored by reversed-phase chromatography.
One Microspin column contains sufficient GalactEXO-coupled agarose beads to hydrolyze β1-3,4-linked galactose on 0.5 mg glycoprotein.
Content and Storage
GalactEXO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
Removal of glycan β-galactose to simplify IgG glycan heterogeneity, improve LC-MS analysis, and reveal underlying glycation modifications.
FAQ and Support
How long is the reaction time for GalactEXO?
For most substrates such as antibodies or Fc-fusion proteins a 2 h incubation results in hydrolysis of over 95% of the present galactose. More complex substrates with high degree of galactosylation may require longer incubation times.
Can you combine GalactEXO with SialEXO or SialEXO 23 to remove both galactoses and sialic acids in a single reaction?
Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.
Does GalactEXO digest fucosylated glycan structures such as Lewis X or Lewis a?
No. Branching fucose linked to the same residue as the β-galactose inhibits GalactEXO activity and needs to be removed prior to GalactEXO treatment. The FucosEXO enzyme can be used for hydrolysis of α1-2, α1-3 and α1-4 linked fucose.
Does GalactEXO hydrolyze galactose on N and O-glycosylated proteins?
Yes, both beta1-3 linked galactose on O-glycans and beta1-4 galactose on N-glycans are hydrolyzed by GalactEXO.
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