€1,048.00 – €1,710.00
Immobilized GalactEXO is an immobilized β-galactosidase for efficient galactose hydrolysis.
Immobilized GalactEXO contains spin columns with immobilized GalactEXO on agarose beads. The immobilized enzymes hydrolyzes β1-3 and β1-4 galactose on N- or O-glycosylated proteins or free oligosaccharides. Immobilized GalactEXO is available as a spin columns for hydrolysis of galactose from 0.5 mg glycoprotein.
| Product Group | |
|---|---|
| Source/Origin | Cloned from A. muciniphila, Produced in E. coli (His-tagged), 87 kDa, 109 kDa |
| Product Composition | |
| Digestion Site | |
| Specificity | Hydrolyzes β1-3 and β1-4 linked galactose on glycoproteins or released glycans |
| Substrates | |
| Reaction Conditions | 30-60 minutes reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
| Buffer Compatibility | Other buffers or pH (6.5 – 9.0) can be used but the reaction conditions need to be optimized. |
| Unit Definition | One column of Immobilized GalactEXO catalyzes the hydrolysis of galactoses from ≥ 95% of 41 μmol 4-nitrophenyl-β-D-galactopyranoside when incubated in 20 mM Tris, 10% ethanol pH 6.8 at room temperature for 15 minutes, monitored by reversed phase chromatography. |
| Application |
Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.
No. Branching fucose linked to the same residue as the β-galactose inhibits GalactEXO activity and needs to be removed prior to GalactEXO treatment. The FucosEXO enzyme can be used for hydrolysis of α1-2, α1-3 and α1-4 linked fucose.
Yes, both beta1-3 linked galactose on O-glycans and beta1-4 galactose on N-glycans are hydrolyzed by GalactEXO.
For most substrates such as antibodies or Fc-fusion proteins a 2 h incubation results in hydrolysis of over 95% of the present galactose. More complex substrates with high degree of galactosylation may require longer incubation times.
