OmniGLYZOR is provided in kits containing material to deglycosylate glycoprotein. Lyophilized PNGase F and RapiGest™ SF Surfactant is provided together with the OmniGLYZOR Microspin columns for optional PNGase F digestion under denaturing conditions
No, both N- and O-glycans are trimmed by the exoglycosidases present in OmniGLYZOR and do not longer represent the structures found on the intact glycoprotein substrate.
OmniGLYZOR hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.
No, it is not recommended. We can only guarantee optimal performance for one-time use.
