OmniGLYZOR®
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OmniGLYZOR® Kit
A mix of immobilized enzymes in spin columns for deglycosylation of glycoproteins carrying N- and simple O-glycans
OmniGLYZOR contains a mixture of immobilized enzymes for fast and efficient removal of N- and simple mucin-type O-glycans on antibodies, fusion proteins and other glycosylated proteins.
Certain N-glycosylation sites are poorly or not accessible to PNGase F unless the substrate protein is denatured. Remaining N-glycans after incubation on the OmniGLYZOR Immobilized Microspin column can be removed by an additional deglycosylation step under denaturing conditions using the PNGase F Lyophilized and RapiGest™ SF surfactant included in the kit.
The enzymes hydrolyzing the O-glycans do not benefit from denaturation which is why the OmniGLYZOR columns should only be used under native conditions.
OmniGLYZOR Kit Microspin is provided in kits containing sufficient material to deglycosylate
5 × 50-100 µg or 10 × 50-100 µg glycoprotein.
Why OmniGLYZOR® Kit?
- Fast and efficient one-pot deglycosylation of N- and O-linked glycans
- Compatible with LC-MS
- Complete removal of glycans for improved analysis of underlying protein
- Immobilized format increases enzyme-to-substrate ratio to enable fast deglycosylation
Available Products
OmniGLYZOR® Kit Microspin 5 x 50-100 ug
A mix of immobilized enzymes in spin columns for deglycosylation of 5 x 50-100 𝜇g glycoprotein carrying N- and simple O-glycans
OmniGLYZOR® Kit Microspin 10 x 50-100 ug
A mix of immobilized enzymes in spin columns for deglycosylation of 10 x 50-100 𝜇g glycoprotein carrying N- and simple O-glycans
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
One OmniGLYZOR Immobilized Microspin column contains agarose beads with immobilized enzymes for deglycosylation of 50-100 µg glycoprotein.
Contents
OmniGLYZOR Kit consists of:
- OmniGlyZOR Immobilized Microspin columns
- PNGase F Lyophilized
- RapiGest™* SF surfactant
Storage
OmniGLYZOR Kit is shipped cold, and the content should be stored at different temperatures upon arrival.
PNGase F Lyophilized and RapiGest SF Surfactant are provided together with the OmniGLYZOR Immobilized Microspin columns for optional PNGase F digestion under denaturing conditions.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
Native removal of O-glycans and removal of inaccessible N-glycans under denaturing conditions, enables complete glycan analysis of the complex C1-inhibitor
Removal of N- and O-glycans from complex and heavily glycosylated proteins, enables clear, interpretable LC-MS analysis.
FAQ and Support
Is it possible to reuse the OmniGLYZOR column?
No, it is not recommended. We can only guarantee optimal performance for one-time use.
Does OmniGLYZOR remove all N-glycans?
OmniGLYZOR hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.
Can OmniGLYZOR be used for released glycan analysis?
No, both N- and O-glycans are trimmed by the exoglycosidases present in OmniGLYZOR and do not longer represent the structures found on the intact glycoprotein substrate.
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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