TrypsINATOR™
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TrypsINATOR™ LysCERATOR Mix Lyophilized
Lyophilized enzyme mix for arginine- and lysine-specific protein digestion
TrypsINATOR (Trypsin) is a serine protease that digests proteins C-terminally of arginine and lysine residues.
TrypsINATOR LysCERATOR Mix Lyophilized is available as a lyophilized powder in vials of 100 μg enzyme.
Why TrypsINATOR™ LysCERATOR Mix Lyophilized?
- Flexible format for method development
- Pre-optimized enzyme mix to maximize digestion efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
TrypsINATOR LysCERATOR Mix Lyophilized 100 ug
Lyophilized enzyme mix for arginine and lysine-specific protein digestion. Processes 2-10 mg protein.
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Quality Control Assay
0.01 μg TrypsINATOR LysCERATOR Mix Lyophilized digests ≥90% of 1 μg apomyoglobin when incubated in 50 mM Tris-HCl pH 8.0 at RT, for 30 minutes.
Buffers
TrypsINATOR LysCERATOR Mix Lyophilized is optimally active in a pH range of 6.0 – 10.0 and compatible with standard digestion buffers, including Tris-HCl, ammonium bicarbonate, HEPES, PBS and TEAB.
Content and Storage
TrypsINATOR LysCERATOR Mix Lyophilized is supplied lyophilized in 50 mM HEPES, 100 mM NaCl, 2% trehalose, pH 8.5, with no preservatives added. It is shipped cold and should be stored at -20°C upon arrival. After reconstitution TrypsINATOR LysCERATOR Mix Lyophilized is stable for 4 weeks at -20°C or for 1 week at +4-8°C.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Applications
Highly specific lysine and arginine digestion with more unique peptides and fewer missed cleavages for confident protein identification in complex proteomics workflows.
Performance Activity
Efficient digestion with minimal missed cleavages and high sequence coverage resulting in efficient peptide mapping and confident protein characterization.
Improved digestion efficiency at challenging K–P sites compared to TrypsINATOR alone, resulting in more complete digestion with fewer missed cleavages.
Robust digestion performance with limited autoproteolysis, enabling cleaner LC–MS data and more confident peptide identification.
FAQ and Support
Are TrypsINATOR and TrypsINATOR LysCERATOR Mix active at lower pH?
Yes, both TrypsINATOR and TrypsINATOR LysCERATOR Mix are active down to pH 5, however, with a lower digestion efficiency.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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