OglyZOR™
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OglyZOR™ Lyophilized
Lyophilized enzyme for hydrolysis of core 1 O-glycans on glycoproteins
OglyZOR is an endoglycosidase (O-Glycosidase) that specifically hydrolyzes core 1 O-glycans on native glycoproteins.
OglyZOR Lyophilized is available as a lyophilized powder in 2000 unit vials for hydrolysis of core 1 O-glycans on 2 mg glycoprotein. It requires removal of sialic aids for activity, and is therefore provided together with 2000 units of SialEXO Lyophilized.
Why OglyZOR™ Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
OglyZOR™ Lyophilized 2000 units
Lyophilized enzyme for hydrolysis of core 1 O-glycans on 2 mg glycoprotein
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
One unit digests ≥ 90% of O-glycans of 1 µg glycoprotein (TNFR) when incubated together with one unit of SialEXO in 20 mM Tris pH 6.8 at 37°C for 2 h.
Contents
OglyZOR Lyophilized includes:
- 1 vial of OglyZOR Lyophilized (G1-OG1-020) supplied in TBS pH 7.6, with no preservatives added.
- 1 vial of SialEXO Lyophilized (G1-SM1-020) supplied in TBS pH 7.6, with no preservatives added.
Storage
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival. After reconstitution, the enzymes are stable for 1 month at +4-8 °C.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »Performance Activity
Hydrolyzes core 1 O-glycans from native glycoproteins, supporting LC-MS workflows for accurate PTM mapping and protein characterization.
Efficient removal of core 1 O-glycans from complex glycoproteins to reduce heterogeneity and improve mass spectrometry characterization.
FAQ and Support
Can I use OglyZOR and PNGaseF in the same reaction?
Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions O/N at pH ~7.0. If denaturing reaction conditions are required for PNGaseF, the reaction with OglyZOR and SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
Will OglyZOR work on extended core 1 structures or single HexNAc?
No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).
Will OglyZOR leave the N-glycans intact?
OglyZOR only works on desialylated O-glycans therefore is a sialidase (SialEXO) included with the product. SialEXO is not O-glycan specific and will therefore desialylate any N-glycans that are present.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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