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Which buffers can be used with the PNGaseF Immobilized column?
The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral pH containing 0.15 M NaCl can also be used but optimization may then be required.
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Can the deglycosylation on the column be performed under denaturing conditions?
The columns are recommended to be used for deglycosylation at native conditions only.
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Is it possible the reuse the PNGAse F Immobilized column?
No, it is not recommended. We can only guarantee optimal performance for one-time use.
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Does PNGase F Immobilized remove all N-linked glycans?
PNGase F Immobilized hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.
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Is it possible to purchase PNGaseF Immobilized in bulk?
Yes! Contact us at info@genovis.com
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