DFO
Site-specific conjugation of IgG with DFO.
GlyCLICK is a site-specific conjugation technology for IgG using Fc glycan remodeling and click-chemistry. The technology generates stable and homogenous antibody conjugates for IgG from several species and subclasses. Fc-glycan remodeling by complete deglycosylation of the antibody allows for site-specific conjugation using robust click-chemistry, resulting in a degree of label (DOL) or drug-antibody ratio (DAR) of 2.
GlyCLICK DFO generates conjugates carrying deferoxamine (DFO), a chelator used for metal-tagging or radiolabeling of IgG for in vivo and in vitro imaging. In contrast to randomly conjugated antibody PET-tracers, site-specific labeling at the Fc glycan sites using GlyCLICK DFO generates tracers that exhibit increased target-affinity, improved stability and a lower off-target accumulation.
GlyCLICK DFO is available for site-specific labeling of 250 μg or 2 mg IgG with the metal chelator DFO (deferoxamine), and is suitable for in vivo and in vitro radiolabeling and imaging.
- Site-specific DFO-conjugation to IgG with a degree of labeling (DOL) of 2
- Facilitates radiolabeling for PET/SPECT imaging
- Improves in vivo imaging quality
Available Products
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
PET/SPECT Imaging
Unit Definition
GlyCLICK DFO contains sufficient material for labeling of 250 μg or 2 mg IgG. For larger amounts or evaluation of the technology, please contact us.
Content and Storage
Components in GlyCLICK DFO:
- GlycINATOR Immobilized
- UDP-GalNAz
- GalT(Y298L)
- sDIBO (DFO)
- All buffers needed
- Concentration and desalting columns
Content should be stored at different temperatures upon arrival.
Instructions
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
The GlyCLICK technology can be used to conjugate IgG with Desferrioxamine (DFO) using the GlyCLICK DFO kit. The DFO is a chelating agent for radiolabeling with the radioisotope Zirconium-89 (89Zr) for PET-imaging. The Azide Activation kit can be used to conjugate a chelator of choice if it is alkyne-modified (carrying for example DIBO, DBCO or BCN) to be compatible with the click chemistry.
The final step of GlyCLICK labeling kit is based on a copper free click reaction (SPAAC) and it is a strain promoted reaction between an azide and an alkyne-carrying label. As the name indicates the reaction occurs spontaneously due to the strained bond angles. The use of this click reaction that forms stable conjugates, allows you to freely choose label or payload to combine with your IgG as long as it is alkyne-functionalized. Several alkyne-carrying labels are commercially available with cyclooctyne structures such as DIBO, DBCO or BCN.
Characterization using LC-MS will give a complete and clear picture of mass shift associated with successful conjugation if the antibody is analyzed reduced or fragmented. LC or LC-MS analysis of HC from a reduced sample or scFc fragments generated by FabRICATOR digestion provides distinct elution peaks for conjugated scFc or mass shifts corresponding to the degree of labelling (DOL) following conjugation. On the application page you can see an example of such analysis. Analysis of fluorescently labeled antibodies can also be performed by absorbance measurements and calculate the DOL using the Extinction coefficient for the antibody and the label and the correction factor for the label.
All the steps (deglycosylation, azide activation and click reaction) in GlyCLICK are highly efficient. Normally, all molecules are conjugated when using GlyCLICK, resulting in DOL=2. On the application page we have shown by MS-analysis that all molecules have been conjugated and there is no remaining un-conjugated material.
Support