TransGLYCIT®
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TransGLYCIT® Remodeling
Glycan remodeling of human IgG with the G0, G1, G2 or G2S2 glycoform
TransGLYCIT is a platform technology that enables efficient and site-specific human IgG glycan remodeling. With TransGLYCIT, antibodies with defined and homogenous glycoforms are generated using fast and robust enzymatic workflows.
TransGLYCIT Remodeling is a platform technology for efficient transglycosylation of native human IgG in a few hours. The robust workflow transglycosylates the antibody Fc N-glycans using enzymatic remodeling, resulting in a homogenous pool of antibodies carrying a defined glycoform. The technology is available in kits to generate antibodies carrying the G0, G1, G2 or G2S2 glycan profiles.
TransGLYCIT Remodeling contains sufficient material to transglycosylate 1 mg human IgG. The glycan-remodeled sample will have the same degree of fucosylation as the original molecule. If you wish to remove core fucosylation, we recommend using TransGLYCIT Remodeling Afucosylated.
Why TransGLYCIT® Remodeling?
- Fast and efficient generation of human IgG with homogeneous glycoforms
- Facilitates functional analysis of specific glycan structures
- Reliable glycan remodeling achieved within a few hours
Available Products
TransGLYCIT® Remodeling G0 1 mg
Glycan remodeling of 1 mg human IgG with the G0 glycoform
TransGLYCIT® Remodeling G1 1 mg
Glycan remodeling of 1 mg human IgG with the G1 glycoform
TransGLYCIT® Remodeling G2 1 mg
Glycan remodeling of 1 mg human IgG with the G2 glycoform
TransGLYCIT® Remodeling G2S2 1 mg
Glycan remodeling of 1 mg human IgG with the G2S2 glycoform
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
TransGLYCIT transglycosylates ≥ 95% of 1 mg human IgG1 as measured by LC-MS.
Contents
TransGLYCIT Remodeling contains:
- GlycINATOR Immobilized Microspin
- TransINATOR enzyme
- Oxazoline glycoform G0, G1, G2 or G2S2
- CaptureSelect™* Fc(ms) Microspin column for purification
Storage
TransGLYCIT Remodeling is shipped cold, and the content should be stored at different temperatures upon arrival.
Do not freeze the GlycINATOR Immobilized or the CaptureSelect™ columns!
Resources
TransGLYCIT® to Evaluate Fc Effector Function of Therapeutic Antibodies
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TransGLYCIT® for Fc-specific N-glycan Remodeling of Human IgG
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Applications
Functional comparison of antibody glycoforms by combining glycoengineering with ADCC and ADCP reporter assays to assess Fc effector activity.
Performance Activity
Simple and rapid generation of IgG with homogeneous G0F or G0 glycoforms for structural and functional characterization.
Rapid generation of galactosylated IgG with or without core fucose for structural and functional characterization.
Rapid generation of sialylated IgG Fc-glycan profiles, with or without core fucose, for structural and functional characterization.
FAQ and Support
What antibody species can I transglycosylate using TransGLYCIT?
The TransGLYCIT platform is developed for transglycosylation of human IgG.
Does TransGLYCIT work on any glycoprotein?
No, TransGLYCIT is based on IgG specific enzymes and will only transglycosylate IgG.
What is the substrate specificity of GlycINATOR?
GlycINATOR is an IgG specific endoglycosidase that hydrolyzes complex, hybrid and high mannonse type glycans on the conserved Fc site on IgG.
Does TransGLYCIT work on all human IgG subclasses?
Yes, the transglycosylation reaction can be performed on all subclasses of human IgG. The reaction is somewhat slower on IgG2 and longer incubation times may be necessary to obtain over 95% transglycosylation.
Can I use the FucosEXO 16 enzyme on native antibodies to remove the core fucose?
Unfortunately no, the enzyme requires trimming of the Fc-glycan using GlycINATOR to enable access to the core fucose substrate.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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