TransGLYCIT®
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TransGLYCIT® Remodeling Afucosylated
Glycan remodeling of human IgG with the G0, G1, G2, G2S2 or Man5 glycoform, with core afucosylation.
TransGLYCIT is a platform technology that enables efficient and site-specific human IgG glycan remodeling. With TransGLYCIT, antibodies with defined and homogenous glycoforms are generated using fast and robust enzymatic workflows.
TransGLYCIT Remodeling Afucosylated is a platform technology for efficient transglycosylation of native human IgG in a few hours. The robust workflow transglycosylates the antibody Fc N-glycans using enzymatic remodeling, resulting in a homogenous pool of antibodies carrying a defined glycoform (G0, G1, G2, G2S2 or Man5) with core afucosylation.
TransGLYCIT Remodeling Afucosylated is provided in kits containing sufficient material to transglycosylate 1 mg human IgG.
Why TransGLYCIT® Remodeling Afucosylated?
- Fast and efficient generation of human IgG with fully afucosylated homogeneous glycoforms
- Complete removal of core fucose to investigate its impact in functional assays
- Reliable glycan remodeling achieved within a few hours
Available Products
TransGLYCIT® Remodeling Afucosylated G0 1 mg
Glycan remodeling of 1 mg human IgG with the G0 glycoform and core afucosylation
TransGLYCIT® Remodeling Afucosylated G1 1 mg
Glycan remodeling of 1 mg human IgG with the G1 glycoform and core afucosylation
TransGLYCIT® Remodeling Afucosylated G2 1 mg
Glycan remodeling of 1 mg human IgG with the G2 glycoform and core afucosylation
TransGLYCIT® Remodeling Afucosylated G2S2 1 mg
Glycan remodeling of 1 mg human IgG with the G2S2 glycoform and core afucosylation
TransGLYCIT® Remodeling Afucosylated Man5 1 mg
Glycan remodeling of 1 mg human IgG with the Man5 glycoform and core afucosylation
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
TransGLYCIT transglycosylates ≥ 95% of 1 mg human IgG1 as measured by LC-MS.
Contents
TransGLYCIT Remodeling Afucosylated contains:
- GlycINATOR/FucosEXO 16 Immobilized Microspin
- TransINATOR enzyme
- Oxazoline glycoform G0, G1, G2, G2S2 or Man5
- CaptureSelect™* Fc(ms) Microspin column for purification
Storage
TransGLYCIT Remodeling Afucosylated is shipped cold, and the content should be stored at different temperatures upon arrival.
Do not freeze the GlycINATOR/FucosEXO 16 Immobilized or the CaptureSelect™ columns!
Resources
TransGLYCIT® to Evaluate Fc Effector Function of Therapeutic Antibodies
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TransGLYCIT® for Fc-specific N-glycan Remodeling of Human IgG
Download Scientific Poster
Applications
Functional comparison of antibody glycoforms by combining glycoengineering with ADCC and ADCP reporter assays to assess Fc effector activity.
Performance Activity
Simple and rapid generation of IgG with homogeneous G0F or G0 glycoforms for structural and functional characterization.
Rapid generation of galactosylated IgG with or without core fucose for structural and functional characterization.
Rapid and simple generation of IgG with defined Man5 glycoforms for structural and functional characterization.
Rapid generation of sialylated IgG Fc-glycan profiles, with or without core fucose, for structural and functional characterization.
FAQ and Support
What antibody species can I transglycosylate using TransGLYCIT?
The TransGLYCIT platform is developed for transglycosylation of human IgG.
Does TransGLYCIT work on any glycoprotein?
No, TransGLYCIT is based on IgG specific enzymes and will only transglycosylate IgG.
What is the substrate specificity of GlycINATOR?
GlycINATOR is an IgG specific endoglycosidase that hydrolyzes complex, hybrid and high mannonse type glycans on the conserved Fc site on IgG.
Does TransGLYCIT work on all human IgG subclasses?
Yes, the transglycosylation reaction can be performed on all subclasses of human IgG. The reaction is somewhat slower on IgG2 and longer incubation times may be necessary to obtain over 95% transglycosylation.
Can I use the FucosEXO 16 enzyme on native antibodies to remove the core fucose?
Unfortunately no, the enzyme requires trimming of the Fc-glycan using GlycINATOR to enable access to the core fucose substrate.
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