PNGase F
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PNGase F Lyophilized
Lyophilized enzyme for hydrolysis of N-glycans on glycoproteins
PNGase F (Peptide N-glycosidase F) is a glycoamidase hydrolyzing the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high-mannose oligosaccharides.
PNGase F Lyophilized is available as a lyophilized powder in 1000 or 5 x 1000 unit vials for digestion of 1 mg or 5 x 1 mg glycoprotein respectively.
Why PNGase F Lyophilized?
- Flexible format for method development
- Possible to combine enzymes for improved efficiency
- Amenable to automated workflows
- Ready to use – just add water!
Available Products
PNGase F Lyophilized 1000 units
Lyophilized enzyme for hydrolysis of N-glycans on 1 mg glycoprotein
PNGase F Lyophilized 5 x 1000 units
Lyophilized enzyme for hydrolysis of N-glycans on 5 x 1 mg glycoprotein
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Unit Defintion
1 unit of PNGase F removes N-glycans from ≥ 95% of 1 µg etanercept when incubated in TBS buffer (50 mM Tris-HCl, 150 mM NaCl) pH 8.6 at 37°C for 1 h.
Content and Storage
PNGase F Lyophilized is supplied in 50 mM HEPES buffer pH 7.5, with no preservatives added. It is shipped cold and should be stored at -20°C upon arrival. After reconstitution, the enzyme is stable for at least 1 month at +4-8°C.
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »SmartEnzymes™ for the Glycosylation Charcterization of Glycoproteins
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GlyCLICK® for Generation and Analysis of ADCs
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PNGase F for Improved DAR Determination in Randomly Conjugated ADCs
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Applications
Comprehensive N-glycan profiling using released glycan workflow, supporting sensitive detection and monitoring of critical quality attributes.
Complete Fc N-glycan removal under native conditions, enabling simplfied subunit LC-MS analysis of therapeutic antibodies.
FAQ and Support
Can I use FabRICATOR and PNGaseF in the same reaction?
Yes, PNGaseF and FabRICATOR can be used in a one-pot reaction as long as the reaction conditions follow the specifications for the FabRICATOR enzyme. It is important to have native reaction conditions for FabRICATOR to remain active.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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