Genovis » IgASAP Sub1 Lyophilized

Description

IgASAP Sub1 is an IgA1-specific protease that digests human IgA1 at one specific site above the hinge, generating intact and homogeneous Fab and Fc fragments.

Detailed Information

Product Group	IgASAP
Application	Antibody Characterization, Antibody digestion, Antibody Fragmentation
Source/Origin	Cloned from S. oralis, Produced in E. coli (His-tagged), 148 kDa
Product Composition	Lyophilized in TBS, Purified to ≥ 90% homogeneity
Digestion Site	Above Hinge
Specificity	VPSTPP/TPSPST (above the hinge)
Substrates	Human IgA1
Reaction Conditions	1 hour reaction at 37°C according to protocol specifications
Digestion Buffers	PBS (pH 7.4), TBS (pH 7.6)
Buffer Compatibility	Active at pH 6.5 to 8.0, does not require reducing conditions or co-factors for activity and tolerates salt concentrations up to 0.3 M NaCl
Inhibitors	ZnCl2, Sensitive to EDTA, Sensitive to high [NaCl]
Unit Definition	1 unit digests ≥ 90% of 1 µg human IgA1

FAQs

Can the IgASAP Sub1 digestion be performed at lower temperatures?

The optimal activity is at 37°C, but the digestion can also be performed at room temperature using a prolonged incubation time.

Does IgASAP Sub1 digest human IgA1-only?

IgASAP Sub1 may digest IgA1 from other primate species with similar hinge sequences, but this has not been tested. IgASAP Sub1 does not digest IgA2.

Which buffers are compatible with IgASAP Sub1?

The enzyme is active at pH 6.5 to 8.0, tolerates up to 300 mM NaCl, and does not require reducing conditions or co-factors for activity. PBS and TBS buffers are both compatible. Optimization may be required if buffers other than the recommended are used.