GlycINATOR™
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GlycINATOR™ Immobilized
Immobilized enzyme for hydrolysis of all types of Fc N-glycans in spin columns
GlycINATOR (EndoS2) is an IgG-specific endoglycosidase that hydrolyzes all glycoforms present at the Fc N-glycosylation sites.
The GlycINATOR Immobilized spin columns contain the GlycINATOR enzyme covalently coupled to agarose beads, for deglycosylation of Fc N-glycans without contaminating the final preparation with enzyme.
The spin columns are available for deglycosylation of up to 0.5 mg (Microspin), 10 mg (Midispin) or 100 mg (Maxispin) IgG. The smallest columns are available in three different pack sizes, with 2, 5 or 10 columns per pack.
Why GlycINATOR™ Immobilized?
- Easy-to-use spin columns
- Increased enzyme-to-substrate ratio for improved digestion efficiency
- No enzyme in the final sample
Available Products
GlycINATOR Immobilized Microspin 2 x 0.5 mg
Immobilized enzyme for deglycosylation of 2 x 0.5 mg IgG in spin columns
GlycINATOR Immobilized Microspin 5 x 0.5 mg
Immobilized enzyme for deglycosylation of 5 x 0.5 mg IgG in spin columns
GlycINATOR Immobilized Midispin 1-10 mg
Immobilized enzyme for deglycosylation of 1-10 mg IgG in spin columns
GlycINATOR Immobilized Microspin 10 x 0.5 mg
Immobilized enzyme for deglycosylation of 10 x 0.5 mg IgG in spin columns
GlycINATOR Immobilized Maxispin 10-100 mg
Immobilized enzyme for deglycosylation of 10-100 mg IgG in spin columns
For information on how to order, visit Place an Order or contact us directly at order@genovis.com.
Product Information
Activity
One spin column contains sufficient GlycINATOR-coupled agarose beads to deglycosylate 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) IgG.
Content and Storage
GlycINATOR Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!
Resources
Instruction for Use
Product Specifications
Safety Data Sheet
Certificate of Analysis
Find your certificate of analysis »FAQ and Support
Can I re-use the GlycINATOR Immobilized column?
We can only guarantee optimal deglycosylation for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
Can I achieve Fc-specific deglycosylation on IgG with N-linked glycans in the Fab region?
Yes, the GlycINATOR enzyme binds specifically to the Fc region and therefore will not deglycosylate N-linked glycans in the Fab region.
Legal & Disclaimers
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals. All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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