FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at one single amino acid position below the hinge generating Fc and F(ab’)2 fragments. FabRICATOR Z is available as lyophilized powder for digestion of 2 mg of mouse IgG2a and IgG3.
| Application | |
|---|---|
| Product Group | |
| Source/Origin | Cloned form S. equi ssp. zooepidemicus, Produced in E. coli (His-tagged), 36.4 kDa |
| Product Composition | |
| Digestion Site | |
| Specificity | |
| Substrates | |
| Reaction Conditions | 2 hour reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
| Buffer Compatibility | |
| Inhibitors | |
| Unit Definition |
No, FabRICATOR Z only digests mouse IgG2a (….CPAPNLLG / GPSVF.. ) and IgG3 (…CPAPNILG / GPSVF…) at one specific site below the hinge. If Fab fragments are an option, we recommend our FabULOUS enzyme that digest mouse IgG1 above the hinge. FabULOUS requires light reducing conditions for optimal activity. If strong reducing conditions are used, this may likely cause reduction of the interchain disulfides.
Unfortunately, FabRICATOR Z does not digest rat IgG2a. If generating Fab fragments is an option, we suggest using FabULOUS enzyme that digests rat IgG2a. FabULOUS does not have a single specific cleavage site, but a primary digestion site, so there can be more than one digestion site. The digestion site(s) for rat IgG2a using FabULOUS is not determined, but the generated fragments are Fab and Fc.
