Genovis » FabRICATOR Z Fab2 Kit

Description

FabRICATOR Z Fab2 Kit contains FragIT Z spin columns with FabRICATOR Z (IdeZ) enzyme immobilized on agarose beads and CaptureSelect^TM spin columns for affinity capture of Fc fragments. FabRICATOR Z Immobilized digests antibodies or Fc-fusion proteins at one single amino acid position below the hinge generating Fc/2 and F(ab’)2 fragments. CaptureSelect^TM spin columns capture Fc fragments for purification of F(ab’)2 and Fc fragments. FabRICATOR Z Fab2 Kit is available in spin column format for digestion and purification of 0.5 – 2.5 mg of mouse IgG2a and IgG3 without residual enzyme in the final preparation.

Detailed Information

Application	Antibody Characterization, Antibody Fragmentation, Purify F(ab')2 and Fc Fragments
Product Group	FabRICATOR Z
Source/Origin	Cloned form S. equi ssp. zooepidemicus, Produced in E. coli (His-tagged), 36.4 kDa
Product Composition	Immobilized on agarose beads (20% EtOH), CaptureSelect Fc columns included
Digestion Site	Below Hinge
Specificity	CPAPNLLG / GPSVF (below the hinge)
Substrates	Monkey IgG, Mouse IgG, Rabbit IgG, Sheep IgG
Reaction Conditions	2 hour reaction at 37°C according to protocol specifications
Digestion Buffers	PBS (pH 6.5)
Buffer Compatibility	A digestion buffer with 50-150 mM NaCl can be used, but digestion time needs to be increased (2-24 h) with increasing NaCl concentration and pH.
Inhibitors	Sensitive to high [NaCl]
Unit Definition	0.1 ml settled resin digests ≥ 95% of 0.5 mg Mouse IgG2a

FAQs

Can I re-use FabRICATOR Z Immobilized columns?

We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.

Can I release Fc fragments from the CaptureSelect column after isolating the F(ab’)2 fragments?

It is possible to elute the Fc fragments from the CaptureSelect column using a low pH buffer, for example a 0.1M Glycine buffer pH=3.0. The pH must immediately after elution be adjusted to neutral pH by adding 0.1x elution volume of 1M Tris pH=8.0.

FabRICATOR Z Fab2 Kit Microspin 0.5 mg quantity	FabRICATOR Z Fab2 Kit Microspin 0.5 mg (A2-FZ2-005)	€529.00
FabRICATOR Z Fab2 Kit Microspin 5 x 0.5 mg quantity	FabRICATOR Z Fab2 Kit Microspin 5 x 0.5 mg (A2-FZ2-025)	€1,408.00

FabRICATOR Z Fab2 Kit

Description

Detailed Information

FAQs

Can I re-use FabRICATOR Z Immobilized columns?

Can I release Fc fragments from the CaptureSelect column after isolating the F(ab’)2 fragments?

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