Description
FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.
FabRICATOR MagIC
€550.00 – €1,875.00
FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.
Detailed Information
| Application | Antibody Fragmentation, Automated Antibody Fragmentation |
|---|---|
| Product Group | FabRICATOR |
| Source/Origin | Cloned from S. pyogenes, Produced in E. coli (His-tagged), 37.7 kDa |
| Product Composition | Immobilized on magnetic agarose beads (20% EtOH) |
| Digestion Site | Below Hinge |
| Specificity | CPAPELLG / GPSVF (below the hinge) |
| Substrates | Human IgG1, Human IgG2, Human IgG4, Monkey IgG, Rabbit IgG, Rat IgG, Sheep IgG |
| Reaction Conditions | 10-20 minute reaction at 37°C according to protocol specifications |
| Digestion Buffers | PBS (pH 7.4) |
| Buffer Compatibility | Other commonly used buffers at physiological pH (6.0 – 8.0) and ionic strength can also be used. Optimization may then be required. |
| Inhibitors | Iodoacetate 20 mM, Iodoacetamide 1 mM, pH < 5.0 |
| Unit Definition | 20 µl settled resin digests ≥ 95% of 0.02-0.2 mg Human IgG1 in 100 µl sample volume |
