FabRICATOR MagIC

580.001,970.00

FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.

580.00
1,970.00
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Description

FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.

Detailed Information

Application

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Product Group

Source/Origin

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Product Composition

Digestion Site

Specificity

Substrates

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Reaction Conditions

Digestion Buffers

Buffer Compatibility

Inhibitors

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Unit Definition

FAQs

FabRICATOR MagIC is suitable for using in automated systems that can handle magnetic beads. On the applications page you can read about how it’s used in a KingFisher™ workflow.

FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2.

The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.

The protocol for FabRICATOR MagIC is based on using standard PBS pH 7.4 as digestion buffer. FabRICATOR in solution is compatible with most commonly used buffers at pH=6-8 and the same buffers should work for FabRICATOR MagIC, but the incubation time might need to be optimized.

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