Description
FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.
FabRICATOR MagIC
€640.00 – €2,150.00
FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.
Detailed Information
| Application | |
|---|---|
| Product Group | |
| Source/Origin | Cloned from S. pyogenes, Produced in E. coli (His-tagged), 37.7 kDa |
| Product Composition | |
| Digestion Site | |
| Specificity | |
| Substrates | Human IgG1, Human IgG2, Human IgG4, Monkey IgG, Rabbit IgG, Rat IgG, Sheep IgG |
| Reaction Conditions | 10-20 minute reaction at 37°C according to protocol specifications |
| Digestion Buffers | |
| Buffer Compatibility | |
| Inhibitors | |
| Unit Definition | 20 µl settled resin digests ≥ 95% of 0.02-0.2 mg Human IgG1 in 100 µl sample volume |
FAQs
FabRICATOR MagIC is suitable for using in automated systems that can handle magnetic beads. On the applications page you can read about how it’s used in a KingFisher™ workflow.
FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).
In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2.
The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.
The protocol for FabRICATOR MagIC is based on using standard PBS pH 7.4 as digestion buffer. FabRICATOR in solution is compatible with most commonly used buffers at pH=6-8 and the same buffers should work for FabRICATOR MagIC, but the incubation time might need to be optimized.
