FabRICATOR MagIC

550.001,875.00

FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.

550.00
1,875.00
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Description

FabRICATOR MagIC includes magnetic beads with immobilized FabRICATOR for automated parallel antibody digestion.

Detailed Information

Application

Antibody Fragmentation, Automated Antibody Fragmentation

Product Group

FabRICATOR

Source/Origin

Cloned from S. pyogenes, Produced in E. coli (His-tagged), 37.7 kDa

Product Composition

Immobilized on magnetic agarose beads (20% EtOH)

Digestion Site

Below Hinge

Specificity

CPAPELLG / GPSVF (below the hinge)

Substrates

Human IgG1, Human IgG2, Human IgG4, Monkey IgG, Rabbit IgG, Rat IgG, Sheep IgG

Reaction Conditions

10-20 minute reaction at 37°C according to protocol specifications

Digestion Buffers

PBS (pH 7.4)

Buffer Compatibility

Other commonly used buffers at physiological pH (6.0 – 8.0) and ionic strength can also be used. Optimization may then be required.

Inhibitors

Iodoacetate 20 mM, Iodoacetamide 1 mM, pH < 5.0

Unit Definition

20 µl settled resin digests ≥ 95% of 0.02-0.2 mg Human IgG1 in 100 µl sample volume

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