Frequently Asked Questions
Got a question? There’s a good chance we’ve answered it here. Browse our FAQs or use the filters below to find what you're looking for. Couldn't find an answer to your question here? Contact us!
Find your question
Can't find an answer to your question? Contact us!
Search question
Product Category
Product
Are there any known inhibitors of ImpaRATOR?
ImpaRATOR is a metalloprotease and thereby sensitive to chelating agents such as EDTA. Concentrations > 1 mM EDTA results in complete inhibition of the enzyme. In addition, the ImpaRATOR activity is inhibited by reducing agents and detergents.
Learn more about ImpaRATOR™
Can ImpaRATOR be used under denaturing conditions?
Insufficient digestion during non-denaturing conditions can be caused by O-glycosylation sites within the glycoprotein inaccessible to the enzyme. In such cases, we recommend trying the following workflow: reduction, denaturation, carboxymethylation, buffer-exchange, and then digestion with ImpaRATOR. If detergent is added, make sure to include a detergent removal step prior ImpaRATOR digestion.
Learn more about ImpaRATOR™
Does ImpaRATOR cleave at all O-glycosylated sites?
ImpaRATOR has a broad activity towards different O-glycan structures; however, the enzyme has limited activity towards sites with two adjacent O-glycosylated Ser/Thr residues. For complete information about O-glycan sites in glycoprotein substrates containing several sites with two adjacent O-glycosylated Ser/Thr residues, OpeRATOR may be a better option.
Learn more about ImpaRATOR™